1a6i: Difference between revisions
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[[Image:1a6i.gif|left|200px]]<br /><applet load="1a6i" size=" | [[Image:1a6i.gif|left|200px]]<br /><applet load="1a6i" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1a6i, resolution 2.4Å" /> | caption="1a6i, resolution 2.4Å" /> | ||
'''TET REPRESSOR, CLASS D VARIANT'''<br /> | '''TET REPRESSOR, CLASS D VARIANT'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1A6I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Known structural/functional Site: <scene name='pdbsite=TNE:Empty Tetracycline Binding Tunnel'>TNE</scene>. Full crystallographic information is available from [http:// | 1A6I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Known structural/functional Site: <scene name='pdbsite=TNE:Empty+Tetracycline+Binding+Tunnel'>TNE</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A6I OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:29:21 2008'' | ||
Revision as of 10:29, 3 February 2008
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TET REPRESSOR, CLASS D VARIANT
OverviewOverview
The X-ray crystal structure analysis of inducer-free Tet repressor, TetR, at 2.4 A resolution identifies one of two openings of the tunnel-like, binding site as the entrance for the inducer tetracycline-Mg2+, [Mg Tc]+., Recognition and binding of the inducer unleashes conformational changes, leading to the induced state of TetR. In the first step, the C-terminal, turn of alpha-helix 6 unwinds, thereby altering the orientation of, alpha-helix 4. This different orientation of alpha-helix 4 is stabilized, by a series of hydrogen bonds mediated through a chain of eight water, molecules. The alpha-helix 4 connects the DNA-binding domain, (alpha-helices 1 to 3) to the rigid TetR core, and thus regulates gene, expression through its respective orientations.
About this StructureAbout this Structure
1A6I is a Single protein structure of sequence from Escherichia coli. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Conformational changes of the Tet repressor induced by tetracycline trapping., Orth P, Cordes F, Schnappinger D, Hillen W, Saenger W, Hinrichs W, J Mol Biol. 1998 Jun 5;279(2):439-47. PMID:9642048
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