1a5t: Difference between revisions
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[[Image:1a5t.gif|left|200px]]<br /><applet load="1a5t" size=" | [[Image:1a5t.gif|left|200px]]<br /><applet load="1a5t" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1a5t, resolution 2.2Å" /> | caption="1a5t, resolution 2.2Å" /> | ||
'''CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III'''<br /> | '''CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1A5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Known structural/functional Site: <scene name='pdbsite=ZNB:Zn Binding Site'>ZNB</scene>. Full crystallographic information is available from [http:// | 1A5T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Known structural/functional Site: <scene name='pdbsite=ZNB:Zn+Binding+Site'>ZNB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5T OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: zinc finger]] | [[Category: zinc finger]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:29:14 2008'' | ||
Revision as of 10:29, 3 February 2008
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CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III
OverviewOverview
The crystal structure of the delta' subunit of the clamp-loader complex of, E. coli DNA polymerase III has been determined. Three consecutive domains, in the structure are arranged in a C-shaped architecture. The N-terminal, domain contains a nonfunctional nucleotide binding site. The catalytic, component of the clamp-loader complex is the gamma subunit, which is, homologous to delta'. A sequence-structure alignment suggests that, nucleotides bind to gamma at an interdomain interface within the inner, surface of the "C." The alignment is extended to other clamp-loader, complexes and to the RuvB family of DNA helicases, and suggests that each, of these is assembled from C-shaped components that can open and close the, jaws of the "C" in response to ATP binding and hydrolysis.
About this StructureAbout this Structure
1A5T is a Single protein structure of sequence from Escherichia coli with as ligand. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the delta' subunit of the clamp-loader complex of E. coli DNA polymerase III., Guenther B, Onrust R, Sali A, O'Donnell M, Kuriyan J, Cell. 1997 Oct 31;91(3):335-45. PMID:9363942
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