6-deoxyerythronolide B synthase (DEBS): Difference between revisions
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Molecular Playground banner: KS-AT The "builder" and the "gatekeeper". | Molecular Playground banner: KS-AT The "builder" and the "gatekeeper". | ||
<scene name='6-deoxyerythronolide_B_synthase_(DEBS)/Ks-at/2'>TextToBeDisplayed</scene> | |||
<applet load='2HG4' size='[450,338]' frame='true' align='right' | <applet load='2HG4' size='[450,338]' frame='true' align='right' | ||
caption='Structure of the ketosynthase-acyltransferase didomain of module 5 from DEBS. ([[2hg4]])' | caption='Structure of the ketosynthase-acyltransferase didomain of module 5 from DEBS. ([[2hg4]])' '6-deoxyerythronolide_B_synthase_(DEBS)/Ks-at/2'> | ||
The AT domain is responsible for selecting the building blocks which become incorporated into the polyketide chain, most often in the form of malonyl-CoA derivatives. Each polyketide synthase AT is exquisitely selective for one substrate type and the DEBS ATs are selective for (S)-methylmalonyl CoA. In this way it functions as the "gatekeeper", preventing erroneous building blocks from becoming incorporated into the growing chain. the methylmalonyl group is transferred by the AT to the ACP, from where it can become incorporated into the polyketide by the KS domain. | The AT domain is responsible for selecting the building blocks which become incorporated into the polyketide chain, most often in the form of malonyl-CoA derivatives. Each polyketide synthase AT is exquisitely selective for one substrate type and the DEBS ATs are selective for (S)-methylmalonyl CoA. In this way it functions as the "gatekeeper", preventing erroneous building blocks from becoming incorporated into the growing chain. the methylmalonyl group is transferred by the AT to the ACP, from where it can become incorporated into the polyketide by the KS domain. | ||
The KS accepts the "in-progress" polyketide from its upstream module, and then catalyzes the decarboxylative condensation of ACP-bound malonyl CoA derivatives with the in-progress chain. This results in an ACP-bound β-ketothioester which is processed by any other β-carbon tailoring domains present within the module. | The KS accepts the "in-progress" polyketide from its upstream module, and then catalyzes the decarboxylative condensation of ACP-bound malonyl CoA derivatives with the in-progress chain. This results in an ACP-bound β-ketothioester which is processed by any other β-carbon tailoring domains present within the module. | ||
===Acyl Carrier Protein (ACP)=== | ===Acyl Carrier Protein (ACP)=== | ||