Prp24: Difference between revisions
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Prp24 co-immunoprecipitates with free U6 and U4/U6 di-snRNP, indicating that it is closely associated with these particles <ref name="Shannon"/><ref name="Ghetti">PMID:7585243</ref>. Initial investigation of the structure showed that Prp24 very likely binds directly to the 40-43 nucleotides of U6 based on chemical modification of naked U6 snRNA compared to free U6 snRNP<ref name="Jandrositz"/>. Further investigation suggested that Prp24 binds within the 30-56 nucleotide region of free U6, as well as to stem II of U4/U6 in the 39-56 and 67-70 nucleotide regions of U6 <ref name="Ghetti"/>. | Prp24 co-immunoprecipitates with free U6 and U4/U6 di-snRNP, indicating that it is closely associated with these particles <ref name="Shannon"/><ref name="Ghetti">PMID:7585243</ref>. Initial investigation of the structure showed that Prp24 very likely binds directly to the 40-43 nucleotides of U6 based on chemical modification of naked U6 snRNA compared to free U6 snRNP<ref name="Jandrositz"/>. Further investigation suggested that Prp24 binds within the 30-56 nucleotide region of free U6, as well as to stem II of U4/U6 in the 39-56 and 67-70 nucleotide regions of U6 <ref name="Ghetti"/>. | ||
The main function of Prp24 seems to be directly related to formation of the U4/U6 complex, particularly based on the evidence that Prp24 is present in U6 and U4/U6, but not U4/U6.U5 <ref name="Shannon"/><ref name="Ghetti"/><ref name="Jandrositz"/>. Prp24 greatly increases the rate and efficiency of U4/U6 annealing <ref name="Raghunathan">PMID:9452384</ref> and mutations in Prp24 have been shown to prevent the formation of the U4/U6 di-snRNP <ref name="Lygerou">PMID:10022888</ref>. Although the exact mechanism by which Prp24 promotes annealing of U4 and U6 is not known, it has been suggested that Prp24 may stabilize the secondary structure of U6 to allow it to interact with U4 in order to allow formation of U4/U6 <ref name="Vidaver"/>. | The main function of Prp24 seems to be directly related to formation of the U4/U6 complex, particularly based on the evidence that Prp24 is present in U6 and U4/U6, but not U4/U6.U5 <ref name="Shannon"/><ref name="Ghetti"/><ref name="Jandrositz"/>. Prp24 greatly increases the rate and efficiency of U4/U6 annealing <ref name="Raghunathan">PMID:9452384</ref> and mutations in Prp24 have been shown to prevent the formation of the U4/U6 di-snRNP <ref name="Lygerou">PMID:10022888</ref>. Although the exact mechanism by which Prp24 promotes annealing of U4 and U6 is not known, it has been suggested that Prp24 may stabilize the secondary structure of U6 to allow it to interact with U4 in order to allow formation of U4/U6 <ref name="Vidaver"/>. | ||