1clx: Difference between revisions
New page: left|200px<br /> <applet load="1clx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1clx, resolution 1.8Å" /> '''CATALYTIC CORE OF XY... |
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==About this Structure== | ==About this Structure== | ||
1CLX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CLX OCA]]. | 1CLX is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CLX OCA]]. | ||
==Reference== | ==Reference== | ||
Refined crystal structure of the catalytic domain of xylanase A from Pseudomonas fluorescens at 1.8 A resolution., Harris GW, Jenkins JA, Connerton I, Pickersgill RW, Acta Crystallogr D Biol Crystallogr. 1996 Mar 1;52(Pt 2):393-401. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299710 15299710] | Refined crystal structure of the catalytic domain of xylanase A from Pseudomonas fluorescens at 1.8 A resolution., Harris GW, Jenkins JA, Connerton I, Pickersgill RW, Acta Crystallogr D Biol Crystallogr. 1996 Mar 1;52(Pt 2):393-401. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299710 15299710] | ||
[[Category: Cellvibrio japonicus]] | [[Category: Cellvibrio japonicus]] | ||
[[Category: Endo-1,4-beta-xylanase]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Connerton, I.]] | [[Category: Connerton, I.]] | ||
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[[Category: xylanase]] | [[Category: xylanase]] | ||
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Revision as of 13:12, 30 October 2007
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CATALYTIC CORE OF XYLANASE A
OverviewOverview
The three-dimensional structure of native xylanase A from Pseudomonas, flouorescens subspecies cellulosa has been refined at 1.8 A resolution., The space group is P2(1)2(1)2(1) with four molecules in the asymmetric, unit. The final model has an R factor of 0.166 for 103 749 reflections, with the four molecules refined independently. The tertiary structure, consists of an eightfold beta/alpha-barrel, the so-called TIM-barrel fold., The active site is in an open cleft at the carboxy-terminal end of the, beta/alpha-barrel, and the active-site residues are a pair of glutamates, Glu127 on strand 4 and Glu246 on strand 7. Both these catalytic glutamate, residues are found on beta-bulges. An atypically long loop after strand 7, is stabilized by calcium. Unusual features include a non-proline, ... [(full description)]
About this StructureAbout this Structure
1CLX is a [Single protein] structure of sequence from [Cellvibrio japonicus] with CA as [ligand]. Active as [Endo-1,4-beta-xylanase], with EC number [3.2.1.8]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
ReferenceReference
Refined crystal structure of the catalytic domain of xylanase A from Pseudomonas fluorescens at 1.8 A resolution., Harris GW, Jenkins JA, Connerton I, Pickersgill RW, Acta Crystallogr D Biol Crystallogr. 1996 Mar 1;52(Pt 2):393-401. PMID:15299710
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