2clm: Difference between revisions

Revision as of 13:12, 30 October 2007

TRYPTOPHAN SYNTHASE (EXTERNAL ALDIMINE STATE) IN COMPLEX WITH N-(4'-TRIFLUOROMETHOXYBENZOYL)-2-AMINO-1-ETHYLPHOSPHATE (F6F)

OverviewOverview

Allosteric interactions regulate substrate channeling in Salmonella, typhimurium tryptophan synthase. The channeling of indole between the, alpha- and beta-sites via the interconnecting 25 A tunnel is regulated by, allosteric signaling arising from binding of ligand to the alpha-site, and, covalent reaction of l-Ser at the beta-site. This signaling switches the, alpha- and beta-subunits between open conformations of low activity and, closed conformations of high activity. Our objective is to synthesize and, characterize new classes of alpha-site ligands (ASLs) that mimic the, binding of substrates, 3-indole-d-glycerol 3'-phosphate (IGP) or, d-glyceraldehyde 3-phosphate (G3P), for use in the investigation of, alpha-site-beta-site interactions. The new synthesized IGP analogues, contain an ... [(full description)]

About this StructureAbout this Structure

2CLM is a [Protein complex] structure of sequences from [Salmonella typhimurium] with NA, F6F and PLS as [ligands]. Active as [Tryptophan synthase], with EC number [4.2.1.20]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

ReferenceReference

Synthesis and characterization of allosteric probes of substrate channeling in the tryptophan synthase bienzyme complex., Ngo H, Harris R, Kimmich N, Casino P, Niks D, Blumenstein L, Barends TR, Kulik V, Weyand M, Schlichting I, Dunn MF, Biochemistry. 2007 Jul 3;46(26):7713-27. Epub 2007 Jun 9. PMID:17559195

Page seeded by OCA on Tue Oct 30 12:17:22 2007

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 ==About this Structure==
-CLM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with NA, F6F and PLS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CLM OCA]].
+CLM is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]] with NA, F6F and PLS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Tryptophan_synthase Tryptophan synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.20 4.2.1.20]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2CLM OCA]].
 ==Reference==
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 [[Category: Protein complex]]
 [[Category: Salmonella typhimurium]]
+[[Category: Tryptophan synthase]]
 [[Category: Barends, T.R.]]
 [[Category: Blumenstein, L.]]
@@ Line 34: / Line 35: @@
 [[Category: tryptophan biosynthesis]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:02:58 2007''
+''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:17:22 2007''