2c4b: Difference between revisions

Revision as of 13:10, 30 October 2007

INHIBITOR CYSTINE KNOT PROTEIN MCOEETI FUSED TO THE CATALYTICALLY INACTIVE BARNASE MUTANT H102A

OverviewOverview

We present a fusion system suited to determine the crystal structure of, small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot, microprotein, was produced as a soluble fusion to a catalytically inactive, variant of the RNAse barnase in Escherichia coli. Functioning as a, versatile tag, barnase facilitated purification, crystallization and, high-resolution structure determination. Flexibility of the linker region, allows for different relative orientations of barnase and the fusion, partner in two crystallographically independent molecules and may thereby, facilitate crystal packing. Nevertheless, the linker region is well, ordered in both molecules. This system may prove more generally useful to, determine the crystal structure of peptides and small proteins.

About this StructureAbout this Structure

2C4B is a [Single protein] structure of sequence from [Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium] with SO4, 2PE, EDO, MES, UNX, GOL and FMT as [ligands]. Active as [Ribonuclease T(1)], with EC number [3.1.27.3]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

ReferenceReference

Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI., Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW, J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:16337652

Page seeded by OCA on Tue Oct 30 12:15:33 2007

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 ==About this Structure==
-C4B is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens,_momordica_cochinchinensis,_ecballium_elaterium Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium]] with SO4, 2PE, EDO, MES, UNX, GOL and FMT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA]].
+C4B is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_amyloliquefaciens,_momordica_cochinchinensis,_ecballium_elaterium Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium]] with SO4, 2PE, EDO, MES, UNX, GOL and FMT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA]].
 ==Reference==
 Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI., Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW, J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16337652 16337652]
 [[Category: Bacillus amyloliquefaciens, momordica cochinchinensis, ecballium elaterium]]
+[[Category: Ribonuclease T(1)]]
 [[Category: Single protein]]
 [[Category: Heinz, D.W.]]
@@ Line 36: / Line 37: @@
 [[Category: squash inhibitor]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:00:33 2007''
+''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:15:33 2007''