Group:MUZIC:Tritopodin: Difference between revisions
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==TRITOPODIN== | ==TRITOPODIN== | ||
Tritopodin is the third member and less researched of the podin family proteins and is codified by the gene ''SYNPO2L''. Tritopodin also known under the names synaptopodin 2-like, Myopodin-like and CHAP (cytoskeletal heart-enriched actin-associated protein), is found in heart (Claeys et al. 2009) <ref> PMID:19151983 </ref> and skeletal muscle tissue (Beqqali et al. 2010) <ref> PMID:20215401 </ref>. Tritopodin share with Myopodin 33% of homology in the Aminoacid sequence, which makes believe similar functions for both proteins. Tritopodin gene it is form by 5 exons and 2 isoforms are postulated: mCHAPa from exon 1,2,3 and 5, as well as mCHAPb that result from a start-codon in exon 4 and the two derives proteins are of 140kDa (mCHAPa) und 110kDa (mCHAPb). | Tritopodin is the third member and less researched of the podin family proteins and is codified by the gene ''SYNPO2L''. Tritopodin also known under the names synaptopodin 2-like, Myopodin-like and CHAP (cytoskeletal heart-enriched actin-associated protein), is found in heart (Claeys et al. 2009) <ref> PMID:19151983 </ref> and skeletal muscle tissue (Beqqali et al. 2010) <ref> PMID:20215401 </ref>. Tritopodin share with Myopodin 33% of homology in the Aminoacid sequence, which makes believe similar functions for both proteins. Tritopodin gene it is form by 5 exons and 2 isoforms are postulated: mCHAPa from exon 1,2,3 and 5, as well as mCHAPb that result from a start-codon in exon 4 and the two derives proteins are of 140kDa (mCHAPa) und 110kDa (mCHAPb). | ||
==Function== | ==Function== | ||
Tritopodin is expressed in vivo and in vitro in differentiated skeletal- and heart muscle cells (Linnemann 2010; Beqqali et al. 2010). | Tritopodin is expressed in vivo and in vitro in differentiated skeletal- and heart muscle cells (Linnemann 2010; Beqqali et al. 2010). | ||
==Tritopodin interactions== | ==Tritopodin interactions== | ||
Tritopodin as Synaptopodin and Myopodin localizes in the sarcomeric Z-disc, where they interact with α-actinin and Filamin C, verify by CoIPs and yeast two-hybrid assays <ref> PMID:20554076 </ref> (Linnemann et al. 2010) Adult skeletal and heart tissue shows the colocalization from mCHAP with α-actinin in the sarcomeric Z-disc and in the nucleus in embrional Cardiomyocytes (Beqqali et al. 2010). | Tritopodin as Synaptopodin and Myopodin localizes in the sarcomeric Z-disc, where they interact with α-actinin and Filamin C, verify by CoIPs and yeast two-hybrid assays <ref> PMID:20554076 </ref> (Linnemann et al. 2010) Adult skeletal and heart tissue shows the colocalization from mCHAP with α-actinin in the sarcomeric Z-disc and in the nucleus in embrional Cardiomyocytes (Beqqali et al. 2010). | ||
[[Image:Tritopodin.jpg|500x]] | [[Image:Tritopodin.jpg|500x]] | ||