The Bacterial Flagellar Hook: Difference between revisions
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In the original monomer structure paper<ref name="hook1" />, the monomer was fitted into an electron density map of about 10 Å resolution, generated by electron cryomicroscopy from hook filaments of ''Salmonella typhimurium'' (see also<ref name="hook2">PMID: 15657146</ref>). Hook filaments are normally curved (at room temperature), but these hook filaments were rendered straight by cold storage, which facilitated image averaging. The D1 and D2 domains of the monomer were independently docked into the map and then ligated. A model of the curved hook filament was generated using symmetry operations that produced the known radius of curvature. This model was then rotated, producing the simulation shown at right. | In the original monomer structure paper<ref name="hook1" />, the monomer was fitted into an electron density map of about 10 Å resolution, generated by electron cryomicroscopy from hook filaments of ''Salmonella typhimurium'' (see also<ref name="hook2">PMID: 15657146</ref>). Hook filaments are normally curved (at room temperature), but these hook filaments were rendered straight by cold storage, which facilitated image averaging. The D1 and D2 domains of the monomer were independently docked into the map and then ligated. A model of the curved hook filament was generated using symmetry operations that produced the known radius of curvature. This model was then rotated, producing the simulation shown at right. | ||
In 2009, a higher resolution (7.1 Å) electron density map of the straight hook, from cryomicroscopy, was reported<ref name="hook3">PMID: 19913483</ref>. | In 2009, a higher resolution (7.1 Å) electron density map of the straight hook, from cryomicroscopy, was reported by Fujii, Kato and Namba<ref name="hook3">PMID: 19913483</ref>. | ||
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