The Bacterial Flagellar Hook: Difference between revisions
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== Hook Filament Structure == | == Hook Filament Structure == | ||
<table align="right" width="200" ><tr><td rowspan="2"> </td><td>[[Image:Samatey hook copyright nature 2004.gif]]</td></tr><tr><td>Bacterial flagellar hook, rotating simulation, used with permission of [[Fadel A. Samatey Group|Fadel Samatey]].</td></tr></table> | <table align="right" width="200" ><tr><td rowspan="2"> </td><td>[[Image:Samatey hook copyright nature 2004.gif]]</td></tr><tr><td>Bacterial flagellar hook, rotating simulation, used with permission of [[Fadel A. Samatey Group|Fadel Samatey]].</td></tr></table> | ||
In the original monomer structure paper<ref name="hook1" />, the monomer was fitted into an electron density map of about 10 Å resolution, generated by electron cryomicroscopy from hook filaments of ''Salmonella typhimurium''. Hook filaments are normally curved (at room temperature), but these hook filaments were rendered straight by cold storage, which facilitated image averaging. The D1 and D2 domains of the monomer were independently docked into the map and then ligated. A model of the curved hook filament was generated using symmetry operations that produced the known radius of curvature. This model was then rotated, producing the simulation shown at right. | In the original monomer structure paper<ref name="hook1" />, the monomer was fitted into an electron density map of about 10 Å resolution, generated by electron cryomicroscopy from hook filaments of ''Salmonella typhimurium'' (see also<. Hook filaments are normally curved (at room temperature), but these hook filaments were rendered straight by cold storage, which facilitated image averaging. The D1 and D2 domains of the monomer were independently docked into the map and then ligated. A model of the curved hook filament was generated using symmetry operations that produced the known radius of curvature. This model was then rotated, producing the simulation shown at right. | ||
{{Clear}} | {{Clear}} | ||
==Monomer Within the Hook== | ==Monomer Within the Hook== | ||
<applet size='400' frame='true' align='right' scene='The_Bacterial_Flagellar_Hook/Flagellar_hook_3rings_pdb_gz/3'/> | <applet size='400' frame='true' align='right' scene='The_Bacterial_Flagellar_Hook/Flagellar_hook_3rings_pdb_gz/3'/> | ||