Rtp and Tus DNA Binding: Difference between revisions
Jump to navigation
Jump to search
Craig Mooney (talk | contribs) No edit summary |
Craig Mooney (talk | contribs) No edit summary |
||
| Line 8: | Line 8: | ||
RTP is a DNA binding protein from Bacillus Subtilis that uses a helix-loop-helix binding motif. In solution it shows a symmetric structure typical of the winged helix loop helix family, with an unstructured <scene name='Rtp_and_Tus_DNA_Binding/Nterm/1'>N-terminus</scene> end, first alpha helix <scene name='Rtp_and_Tus_DNA_Binding/Alpha1/1'>(a1)</scene>, unstructured loop that is equivalent to the first beta sheet <scene name='Rtp_and_Tus_DNA_Binding/Beta1/1'>(B1)</scene>, helix loop helix structure (<scene name='Rtp_and_Tus_DNA_Binding/Alpha2/1'>a2</scene>-<scene name='Rtp_and_Tus_DNA_Binding/Alpha3/1'>a3</scene>), 2 beta sheets with a connecting loop that makes up the 'wing' structure <scene name='Rtp_and_Tus_DNA_Binding/Wing/1'>(B2 - B3)</scene> and an additional long alpha helix involved in dimerisation <scene name='Rtp_and_Tus_DNA_Binding/Alpha4/1'>(a4)</scene>. | RTP is a DNA binding protein from Bacillus Subtilis that uses a helix-loop-helix binding motif. In solution it shows a symmetric structure typical of the winged helix loop helix family, with an unstructured <scene name='Rtp_and_Tus_DNA_Binding/Nterm/1'>N-terminus</scene> end, first alpha helix <scene name='Rtp_and_Tus_DNA_Binding/Alpha1/1'>(a1)</scene>, unstructured loop that is equivalent to the first beta sheet <scene name='Rtp_and_Tus_DNA_Binding/Beta1/1'>(B1)</scene>, helix loop helix structure (<scene name='Rtp_and_Tus_DNA_Binding/Alpha2/1'>a2</scene>-<scene name='Rtp_and_Tus_DNA_Binding/Alpha3/1'>a3</scene>), 2 beta sheets with a connecting loop that makes up the 'wing' structure <scene name='Rtp_and_Tus_DNA_Binding/Wing/1'>(B2 - B3)</scene> and an additional long alpha helix involved in dimerisation <scene name='Rtp_and_Tus_DNA_Binding/Alpha4/1'>(a4)</scene>. | ||
Assymmetry of RTP in DNA-binding | |||
Wing-up <scene name='Rtp_and_Tus_DNA_Binding/Wingup/1'>(green)</scene>: Contacts upstream with rtp dimer bound to A-site | |||
Wing-down <scene name='Rtp_and_Tus_DNA_Binding/Wingdown/1'>(blue)</scene>: Contacts with phosphate backbone of downstream DNA | |||
<scene name='Rtp_and_Tus_DNA_Binding/Overview/1'>Overview</scene> | <scene name='Rtp_and_Tus_DNA_Binding/Overview/1'>Overview</scene> | ||
Revision as of 06:17, 23 May 2011
Replication Termination ProteinsReplication Termination Proteins
Rtp and Tus These polar fork bocking sites have been found in yeast, pea, frog and human genomes.
RTPRTP
RTP is a DNA binding protein from Bacillus Subtilis that uses a helix-loop-helix binding motif. In solution it shows a symmetric structure typical of the winged helix loop helix family, with an unstructured end, first alpha helix , unstructured loop that is equivalent to the first beta sheet , helix loop helix structure (-), 2 beta sheets with a connecting loop that makes up the 'wing' structure and an additional long alpha helix involved in dimerisation .
Assymmetry of RTP in DNA-binding Wing-up : Contacts upstream with rtp dimer bound to A-site Wing-down : Contacts with phosphate backbone of downstream DNA
|