Sandbox20: Difference between revisions

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Student, Craig T Martin, Michael Webster

Revision as of 03:07, 22 May 2011 view source Michael Webster (talk \| contribs) 118 edits →‎Replication Termination Activity ← Older edit		Revision as of 03:11, 22 May 2011 view source Michael Webster (talk \| contribs) 118 edits →‎Tus Newer edit →
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	Before the structure of the Tus-Ter complex was determined, mutation of Glu49 was shown to eliminate anti-helicase activity without affecting DNA binding. This could not be explained by the [[1ecr\|original crystal structure]] as it is not located close enough to make direct contact with the conserved cytosine. However, the [[2ewj\|more recent structure]] revealed that this is due the water-mediated hydrogen bond formed between it and the adenine residue adjacent to cytosine. It is therefore likely, that the interaction is necessary to compensate for the disrupted H-bonding in the nucleotide adjacent to the displaced cytosine, as shown in this <scene name='Sandbox20/Tus/18'>model</scene>.		Before the structure of the Tus-Ter complex was determined, mutation of Glu49 was shown to eliminate anti-helicase activity without affecting DNA binding. This could not be explained by the [[1ecr\|original crystal structure]] as it is not located close enough to make direct contact with the conserved cytosine. However, the [[2ewj\|more recent structure]] revealed that this is due the water-mediated hydrogen bond formed between it and the adenine residue adjacent to cytosine. It is therefore likely, that the interaction is necessary to compensate for the disrupted H-bonding in the nucleotide adjacent to the displaced cytosine, as shown in this <scene name='Sandbox20/Tus/18'>model</scene>.

			Index of Tus scenes: [1] <scene name='Sandbox20/Tus/2'>Original</scene>, [2] <scene name='Sandbox20/Tus/19'>DNA binding</scene>, [3] <scene name='Sandbox20/Tus/13'>Conserved cytosine</scene>, [4] <scene name='Sandbox20/Tus/9'>Cytosine displacement</scene>, [5] <scene name='Sandbox20/Tus/18'>E49 hydrogen bond</scene>.