Sandbox Reserved 403: Difference between revisions
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== Hc and Ganglioside Interaction == | == Hc and Ganglioside Interaction == | ||
[[Image:TeNT.jpg|thumb|200px| | [[Image:TeNT.jpg|thumb|200px|left|An overlap of three copies of the TeNT Hc structure. This image helps to illustrate the two separte and distinct domains of the Hc fragment of tetanospasmin.<ref name="hcfrag" />]] | ||
Hc has two distinct domains:<ref name="hcfrag"> PMID:11418600</ref> | Hc has two distinct domains:<ref name="hcfrag"> PMID:11418600</ref> | ||
[[Image:GT1-b ganglioside.jpg|thumb|350px|right|Ganglioside GT1-b.<ref name="hcfrag" />]] | |||
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Binding studies have shown that a particular ganglioside, GT1-b, is necessary for the binding of the Hc fragment of tetanospasmin (TeNT). An analogue of the GT1-b ganglioside was made in order to increase solubility because a crystal structure of the Hc and native GT1-b could not be obtained. | Binding studies have shown that a particular ganglioside, GT1-b, is necessary for the binding of the Hc fragment of tetanospasmin (TeNT). An analogue of the GT1-b ganglioside was made in order to increase solubility because a crystal structure of the Hc and native GT1-b could not be obtained. | ||
[[Image:GT1-b | [[Image:GT1-b Ana.jpg|thumb|350px|right|GT1-b analogue which was used to bind to the Hc fragment in this study. The analogue differs from the native GT1-b in that Sia6 is the β-anomer and the ceramide group has been replaced.<ref name="hcfrag" />]] | ||
The Hc fragment has two binding sites in the β-trefoil domain:<ref name="hcfrag" /> | The Hc fragment has two binding sites in the β-trefoil domain:<ref name="hcfrag" /> | ||