2oqn: Difference between revisions
New page: left|200px<br /><applet load="2oqn" size="450" color="white" frame="true" align="right" spinBox="true" caption="2oqn, resolution 1.90Å" /> '''High Pressure Cryoco... |
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[[Image:2oqn.jpg|left|200px]]<br /><applet load="2oqn" size=" | [[Image:2oqn.jpg|left|200px]]<br /><applet load="2oqn" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2oqn, resolution 1.90Å" /> | caption="2oqn, resolution 1.90Å" /> | ||
'''High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths'''<br /> | '''High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2OQN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii] with TAR as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 2OQN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii] with <scene name='pdbligand=TAR:'>TAR</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQN OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: sulfur sad phasing]] | [[Category: sulfur sad phasing]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 15:28:42 2008'' | ||
Revision as of 16:28, 23 January 2008
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High Pressure Cryocooling of Capillary Sample Cryoprotection and Diffraction Phasing at Long Wavelengths
OverviewOverview
Crystal cryocooling is usually employed to reduce radiation damage during, X-ray crystallography. Recently, a high-pressure cryocooling method has, been developed which results in excellent diffraction-quality crystals, without the use of penetrative cryoprotectants. Three new developments of, the method are presented here: (i) Xe-He high-pressure cryocooling for Xe, SAD phasing, (ii) native sulfur SAD phasing and (iii) successful, cryopreservation of crystals in thick-walled capillaries without, additional cryoprotectants other than the native mother liquor. These, developments may be useful for structural solution of proteins without the, need for selenomethionine incorporation and for high-throughput protein, crystallography.
About this StructureAbout this Structure
2OQN is a Single protein structure of sequence from Thaumatococcus daniellii with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
High-pressure cryocooling for capillary sample cryoprotection and diffraction phasing at long wavelengths., Kim CU, Hao Q, Gruner SM, Acta Crystallogr D Biol Crystallogr. 2007 May;63(Pt 5):653-9. Epub 2007, Apr 21. PMID:17452791
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