Sandbox Reserved 196: Difference between revisions
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== Structure and Biology of RNase B == | == Structure and Biology of RNase B == | ||
[[Image: | [[Image:1RBJ.jpg | thumb|left|RNase B]] | ||
Crystallization of RNase A and RNase B has shown that these two enzymes are identical in their<Structure load='1rbj' size='250' frame='true' align='right' caption='Molecule II of Ribonuclease B with a strand of DNA in active site' scene='Sandbox_Reserved_196/Secondary_structure/13' name ='1rbj' /> primary structure and amino acid makeup; however, RNase B has a single glycosylation at the <scene name='Sandbox_Reserved_196/Rbb_basic/18'>Asn-34</scene> site that differentiates RNase B from RNase A. RNase B has from five to nine mannose residues attached which can create a variance within RNase B molecules. This glycosylation increases the kinetic stability of the RNase B by 3 kJ/mol<ref name="fourth">PMID:10600722</ref>. This addition to RNase B, however, does not significantly change the protein conformation from RNase A<ref name="fifth">PMID:1322837</ref>. | Crystallization of RNase A and RNase B has shown that these two enzymes are identical in their<Structure load='1rbj' size='250' frame='true' align='right' caption='Molecule II of Ribonuclease B with a strand of DNA in active site' scene='Sandbox_Reserved_196/Secondary_structure/13' name ='1rbj' /> primary structure and amino acid makeup; however, RNase B has a single glycosylation at the <scene name='Sandbox_Reserved_196/Rbb_basic/18'>Asn-34</scene> site that differentiates RNase B from RNase A. RNase B has from five to nine mannose residues attached which can create a variance within RNase B molecules. This glycosylation increases the kinetic stability of the RNase B by 3 kJ/mol<ref name="fourth">PMID:10600722</ref>. This addition to RNase B, however, does not significantly change the protein conformation from RNase A<ref name="fifth">PMID:1322837</ref>. | ||