Sandbox Reserved 195: Difference between revisions
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RNase A and RNase S have very similar structures except for a few key areas, one being the cleavage site (residues 16-23)<ref name="Raines"> PMID:11848924</ref>. Decreased order in these amino acids is seen in RNase S. The <scene name='Sandbox_Reserved_195/Clevage_site/1' target='1'>cleavage site</scene> on RNase A is located between residues Ala 20 and Ser 21. Upon cleavage, the fragments S peptide and S protein are created, giving RNase S its unique structure. <scene name='Sandbox_Reserved_195/S_peptide/1' target='0'>S Peptide</scene> consists of residues 1-20, and is largely responsible for proper folding. S protein is comprised of residues 21-124. Additionally, RNase A and RNase S have many conserved structural components, as they are essentially the same protein. Glutamine 60 is a highly conserved amino acid throughout many different species, showing its importance in both <scene name='Sandbox_Reserved_195/Gln_60/2' target='0'>RNase S</scene> and <scene name='Sandbox_Reserved_195/Glutamine_60_rnase_a/1' target='1'>RNase A</scene>. Glu 60 is also interesting, because it is the only residue in an unfavorable position (Φ= -100, ψ= -130) as defined in the Ramachandran plot <ref name="Kim">PMID:1463719</ref>. | RNase A and RNase S have very similar structures except for a few key areas, one being the cleavage site (residues 16-23)<ref name="Raines"> PMID:11848924</ref>. Decreased order in these amino acids is seen in RNase S. The <scene name='Sandbox_Reserved_195/Clevage_site/1' target='1'>cleavage site</scene> on RNase A is located between residues Ala 20 and Ser 21. Upon cleavage, the fragments S peptide and S protein are created, giving RNase S its unique structure. <scene name='Sandbox_Reserved_195/S_peptide/1' target='0'>S Peptide</scene> consists of residues 1-20, and is largely responsible for proper folding. S protein is comprised of residues 21-124. Additionally, RNase A and RNase S have many conserved structural components, as they are essentially the same protein. Glutamine 60 is a highly conserved amino acid throughout many different species, showing its importance in both <scene name='Sandbox_Reserved_195/Gln_60/2' target='0'>RNase S</scene> and <scene name='Sandbox_Reserved_195/Glutamine_60_rnase_a/1' target='1'>RNase A</scene>. Glu 60 is also interesting, because it is the only residue in an unfavorable position (Φ= -100, ψ= -130) as defined in the Ramachandran plot <ref name="Kim">PMID:1463719</ref>. | ||
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The <scene name='Sandbox_Reserved_195/Active_site/1' target='0'>active site</scene> of RNase S consists of residues His 12, Lys 41, Val 43, Asn 44, Thr 45, His 119, Phe 120, Asp 121, and Ser 123. Residues 1, 15-20, 21-23, and 124 could be removed without serious consequence to the structure or activity of RNase S. Residue 124 could also be removed from RNase A without compromising the structure. <ref name="Wyckoff et al">PMID:6037556</ref> | The <scene name='Sandbox_Reserved_195/Active_site/1' target='0'>active site</scene> of RNase S consists of residues His 12, Lys 41, Val 43, Asn 44, Thr 45, His 119, Phe 120, Asp 121, and Ser 123. Residues 1, 15-20, 21-23, and 124 could be removed without serious consequence to the structure or activity of RNase S. Residue 124 could also be removed from RNase A without compromising the structure. <ref name="Wyckoff et al">PMID:6037556</ref> | ||
<scene name='Sandbox_Reserved_195/Asp_14/2'>Asp 14</scene> | |||
<Structure load='7rsa' size='500' frame='true' align='left' caption='RNase A (PDB: 7rsa), also known as bovine pancreatic ribonuclease A, has been studied for years to learn about protein structure, stability, and folding.' scene='Insert optional scene name here' target='1' /> | <Structure load='7rsa' size='500' frame='true' align='left' caption='RNase A (PDB: 7rsa), also known as bovine pancreatic ribonuclease A, has been studied for years to learn about protein structure, stability, and folding.' scene='Insert optional scene name here' target='1' /> | ||
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RNase S on PDB: http://www.rcsb.org/pdb/explore/explore.do?structureId=1RNU | RNase S on PDB: http://www.rcsb.org/pdb/explore/explore.do?structureId=1RNU | ||
RNase A on PDB: | RNase A on PDB: http://www.rcsb.org/pdb/explore/explore.do?structureId=7RSA | ||
Ribonuclease on Proteopedia: http://proteopedia.org/wiki/index.php/Ribonuclease | Ribonuclease on Proteopedia: http://proteopedia.org/wiki/index.php/Ribonuclease | ||