Sandbox Reserved 195: Difference between revisions

RNase A and RNase S have very similar structures except for a few key areas, one being the cleavage site (residues 16-23)<ref name="Raines"> PMID:11848924</ref>.  Decreased order in these amino acids is seen in RNase S.  The <scene name='Sandbox_Reserved_195/Clevage_site/1' target='1'>cleavage site</scene> on RNase A is located between residues Ala 20 and Ser 21.  Upon cleavage, the fragments S peptide and S protein are created, giving RNase S its unique structure.  <scene name='Sandbox_Reserved_195/S_peptide/1' target='0'>S Peptide</scene> consists of residues 1-20, and is largely responsible for proper folding.  S protein is comprised of residues 21-124.  Additionally, RNase A and RNase S have many conserved structural components, as they are essentially the same protein.  Glutamine 60 is a highly conserved amino acid throughout many different species, showing its importance in both <scene name='Sandbox_Reserved_195/Gln_60/2' target='0'>RNase S</scene> and <scene name='Sandbox_Reserved_195/Glutamine_60_rnase_a/1' target='1'>RNase A</scene>.  Glu 60 is also interesting, because it is the only residue in an unfavorable position (Φ= -100, ψ= -130) as defined in the Ramachandran plot <ref name="Kim">PMID:1463719</ref>.