Sandbox Reserved 195: Difference between revisions

Jump to navigation Jump to search
No edit summary
No edit summary
Line 43: Line 43:


Hydrogen bonding and hydrophobic interactions play a major role in the structure of RNase S. <ref name="Kim" /> There are 84 water molecules present, with 8 of these specifically connecting S peptide and S protein.  Some of these water molecules are also conserved throughout all RNase derivatives.  Hydrophobic interactions between residues Phe 8, Met 13, His 12, Ala 4 are essential in holding the S Peptide in place.  In addition to these residues, Asp 14 is also important in peptide-protein binding, as represented in the 2D picture.  In the picture, it appears that the protein and peptide are not connected; this is because the bond cleavage between residues 20 and 21 of RNase A has already occurred.  Additionally RNase S has a rigid hydrophobic core; one-third of the surface of the core is made up of the S peptide.  The surrounding loops have more flexibility.   
Hydrogen bonding and hydrophobic interactions play a major role in the structure of RNase S. <ref name="Kim" /> There are 84 water molecules present, with 8 of these specifically connecting S peptide and S protein.  Some of these water molecules are also conserved throughout all RNase derivatives.  Hydrophobic interactions between residues Phe 8, Met 13, His 12, Ala 4 are essential in holding the S Peptide in place.  In addition to these residues, Asp 14 is also important in peptide-protein binding, as represented in the 2D picture.  In the picture, it appears that the protein and peptide are not connected; this is because the bond cleavage between residues 20 and 21 of RNase A has already occurred.  Additionally RNase S has a rigid hydrophobic core; one-third of the surface of the core is made up of the S peptide.  The surrounding loops have more flexibility.   




Line 55: Line 56:


[[Image:surface view of rnase s.png]]
[[Image:surface view of rnase s.png]]
[[Image:asp14.png]]
                                                                                                                         {{Quote box
                                                                                                                         {{Quote box
  | quote  = Residue Asp 14 which aids in S peptide - S protein bond formation.
  | quote  = The image above depicts the surface interaction between the S peptide and S protein fragments; S peptide is blue, S protein is yellow.
  | source =
  | source =
  | width  =10
  | width  =10

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA, Melanie Clark, Ashton Chaffee, R. Jeremy Johnson