Non-polymerizable monomeric actin: Difference between revisions
No edit summary |
No edit summary |
||
| Line 3: | Line 3: | ||
Non-polymerizable monomeric actin or AP-actin is an Sf9-expressed cytoplasmic actin harboring two point mutations that prevent the monomer from polymerizing into actin filaments. These mutations allow for the crystallization of actin without the use of specific toxins or actin-binding proteins that may influence the structure. The crystal structure of AP-actin has been solved for the ADP-bound form ([[2HF3]]) and the ATP-bound form ([[2HF4]]). These two structures are shown below as a morph between the two states. | Non-polymerizable monomeric actin or AP-actin is an Sf9-expressed cytoplasmic actin harboring two point mutations that prevent the monomer from polymerizing into actin filaments. These mutations allow for the crystallization of actin without the use of specific toxins or actin-binding proteins that may influence the structure. The crystal structure of AP-actin has been solved for the ADP-bound form ([[2HF3]]) and the ATP-bound form ([[2HF4]]). These two structures are shown below as a morph between the two states. | ||
===Structural features of actin=== | |||
The actin monomer, or G-actin, contains four structural domains. Domain 1 includes amino acids 1-32, 70-144 and 138-375. Domain 2, amino acids 33-69. Domain 3, amino acids 145-180 and 270-337 and domain 4, amino acids 181-269. | |||
Revision as of 03:54, 12 April 2011
Non-polymerizable monomeric actin or AP-actin is an Sf9-expressed cytoplasmic actin harboring two point mutations that prevent the monomer from polymerizing into actin filaments. These mutations allow for the crystallization of actin without the use of specific toxins or actin-binding proteins that may influence the structure. The crystal structure of AP-actin has been solved for the ADP-bound form (2HF3) and the ATP-bound form (2HF4). These two structures are shown below as a morph between the two states.
Structural features of actinStructural features of actin
The actin monomer, or G-actin, contains four structural domains. Domain 1 includes amino acids 1-32, 70-144 and 138-375. Domain 2, amino acids 33-69. Domain 3, amino acids 145-180 and 270-337 and domain 4, amino acids 181-269.
Crystal structure of AP-actin in the ATP and ADP stateCrystal structure of AP-actin in the ATP and ADP state
| |||||||
| Morph of the lac repressor complexed with DNA showing the differences between non-specific binding (straight DNA) vs. specific recognition of the operator sequence (kinked DNA). Whether the binding kinks the DNA, or simply stabilizes a pre-existing kink, is unknown. Details. |
Structural features of AP-actinStructural features of AP-actin
This is text. This is text. This is text. This is text. This is text. This is text. This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.This is text. This is text. This is text. This is text. This is text. This is text.
MechanismMechanism
About this StructureAbout this Structure
This is where I will add my text. 2hf4 is a 1 chain structure of Actin with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
See AlsoSee Also
ReferenceReference
- ↑ Rould MA, Wan Q, Joel PB, Lowey S, Trybus KM. Crystal structures of expressed non-polymerizable monomeric actin in the ADP and ATP states. J Biol Chem. 2006 Oct 20;281(42):31909-19. Epub 2006 Aug 18. PMID:16920713 doi:M601973200