Non-polymerizable monomeric actin: Difference between revisions
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Non-polymerizable monomeric actin or AP-actin is an Sf9-expressed cytoplasmic actin harboring two point mutations that prevent the monomer from polymerizing into actin filaments. These mutations allow for the crystallization of actin without the use of specific toxins or actin-binding proteins that may influence the structure. The crystal structure of AP-actin has been solved for the ADP-bound form (PDB code 2HF4) and the ATP-bound form (PDB code 2HF3). These two structures are shown right as a morph between the two states. | Non-polymerizable monomeric actin or AP-actin is an Sf9-expressed cytoplasmic actin harboring two point mutations that prevent the monomer from polymerizing into actin filaments. These mutations allow for the crystallization of actin without the use of specific toxins or actin-binding proteins that may influence the structure. The crystal structure of AP-actin has been solved for the ADP-bound form (PDB code 2HF4) and the ATP-bound form (PDB code 2HF3). These two structures are shown right as a morph between the two states. | ||
===Crystal structure of Monomeric Actin in its ATP-bound state=== | |||
<table width='450' align='left' cellpadding='5'><tr><td rowspan='2'> </td><td bgcolor='#d0d0d0'><applet load=Image:2HF3 2HF4 10state morph4.pdb' size='450' align='right' scene='User:Thomas_E_Sladewski/Sandbox_1/10state_morph_scene1/1' /></td></tr><tr><td bgcolor='#d0d0d0'>Morph of the [[lac repressor]] complexed with DNA showing the differences between non-specific binding (straight DNA) vs. specific recognition of the operator sequence (kinked DNA). Whether the binding kinks the DNA, or simply stabilizes a pre-existing kink, is unknown. [[Lac repressor#Specific Binding|Details]].</td></tr></table> | |||
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