User:Michael Patrick/Sandbox 2: Difference between revisions

Line 22:

===STRUCTURE===

GFP has a typical beta barrel consisting of <scene name='User:Michael_Patrick/Sandbox_2/Ema-0/4'>one β-sheet</scene> with <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/2'>alpha helix(s) containing the chromophore running through the center.</scene> Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65–Tyr66–Gly67 that lead to chromophore formation. This process of post-translational modification is referred to as maturation. The hydrogen-bonding network and electron-stacking interactions with these sidechains influence the color of wtGFP and its numerous derivatives. The tightly packed nature of the barrel excludes solvent molecules, protecting the chromophore fluorescence from quenching by water.

GFP has a typical beta barrel consisting of <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/4'>one β-sheet</scene> with <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/3'>alpha helix(s) containing the chromophore running through the center.</scene> Inward-facing sidechains of the barrel induce specific cyclization reactions in the <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/5'>tripeptide Ser65–Tyr66–Gly67</scene> that lead to chromophore formation. This process of post-translational modification is referred to as maturation. The hydrogen-bonding network and electron-stacking interactions with these sidechains influence the color of wtGFP and its numerous derivatives.

<scene name='User:Michael_Patrick/Sandbox_2/Ema-3/6'>The tightly packed nature of the barrel</scene> excludes solvent molecules, protecting the chromophore fluorescence from quenching by water.

@@ Line 22: / Line 22: @@
 ===STRUCTURE===
 <Structure load='1ema' size='500' frame='true' align='right' caption='GFP in backbone format and spacefill chromophore' scene='User:Michael_Patrick/Sandbox_2/Ema-1/2' />
-GFP has a typical beta barrel consisting of <scene name='User:Michael_Patrick/Sandbox_2/Ema-0/4'>one β-sheet</scene> with <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/2'>alpha helix(s) containing the chromophore running through the center.</scene> Inward-facing sidechains of the barrel induce specific cyclization reactions in the tripeptide Ser65–Tyr66–Gly67 that lead to chromophore formation. This process of post-translational modification is referred to as maturation. The hydrogen-bonding network and electron-stacking interactions with these sidechains influence the color of wtGFP and its numerous derivatives. The tightly packed nature of the barrel excludes solvent molecules, protecting the chromophore fluorescence from quenching by water.
+GFP has a typical beta barrel consisting of <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/4'>one β-sheet</scene> with <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/3'>alpha helix(s) containing the chromophore running through the center.</scene> Inward-facing sidechains of the barrel induce specific cyclization reactions in the <scene name='User:Michael_Patrick/Sandbox_2/Ema-3/5'>tripeptide Ser65–Tyr66–Gly67</scene> that lead to chromophore formation. This process of post-translational modification is referred to as maturation. The hydrogen-bonding network and electron-stacking interactions with these sidechains influence the color of wtGFP and its numerous derivatives.
+<scene name='User:Michael_Patrick/Sandbox_2/Ema-3/6'>The tightly packed nature of the barrel</scene> excludes solvent molecules, protecting the chromophore fluorescence from quenching by water.