Sandbox Reserved 313: Difference between revisions
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''Alkaline sphingomyelinase (bSMase)'' - Alkaline SMase is found in the intestine and hydrolyses sphingomyelin in both the lumen and the mucosal membrane and requires bile salts for activity. This enzyme shares some similarities with the nucleotide pyrophosphatase(NPP) family and is called NPP7. The enzyme has a hydrophobic domain at the N and C terminus, where the N terminus acts as a signal peptide (which is eventually cleaved) and the C terminus acts as a signal anchor which attaches the enzyme to membranes<ref name="gp3">PMID: 12401200 </ref><ref name="gp8">PMID: 16631405 </ref>. | ''Alkaline sphingomyelinase (bSMase)'' - Alkaline SMase is found in the intestine and hydrolyses sphingomyelin in both the lumen and the mucosal membrane and requires bile salts for activity. This enzyme shares some similarities with the nucleotide pyrophosphatase(NPP) family and is called NPP7. The enzyme has a hydrophobic domain at the N and C terminus, where the N terminus acts as a signal peptide (which is eventually cleaved) and the C terminus acts as a signal anchor which attaches the enzyme to membranes<ref name="gp3">PMID: 12401200 </ref><ref name="gp8">PMID: 16631405 </ref>. | ||
''Bacterial sphingomyelinase'' - | ''Bacterial sphingomyelinase'' - This enzyme is secreted from a bacterial cell and it has been shown that both bacterial SMase and mammalian DNase I catalyze the hydrolysis of the phosphodiester bonds of substrates which depends on the divalent metal ions present. both bacterial SMase and mammalian DNase I have a highly conserved pentapeptide sequence SDHYP which include the catalytically important residues Asp 251 and His 252<ref name="gp9">PMID: 8976554 </ref>. | ||
=Structure and Function= | =Structure and Function= | ||