Sandbox Reserved 301: Difference between revisions

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<ref name="Abad">PMID:12196524</ref>
<ref name="Abad">PMID:12196524</ref>
<ref name="Abad"/>
<ref name="Abad"/>
Escherichia coli Branching Enzyme (BE) (1,4-a-glucan 6-glucosyltransferase)catalyzes the formation of a-1,6 branch points of glycogen (similar process for start with starch branching points). The enzyme contains three domains: a NH2-terminal seven stranded b-sandwich domain, a COOH-terminal domain, and a central a/b-barrel domain containing the enzyme active site. The branching enzyme belongs to the a-amylase family of enzymes.
Escherichia coli Branching Enzyme (BE) (1,4-a-glucan 6-glucosyltransferase)catalyzes the formation of a-1,6 branch points of glycogen. The enzyme contains three domains: a NH2-terminal seven stranded b-sandwich domain, a COOH-terminal domain, and a central a/b-barrel domain containing the enzyme active site. The branching enzyme belongs to the a-amylase family of enzymes.
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<scene name='Sandbox_Reserved_301/Gbe1/2'>TextToBeDisplayed</scene>
<scene name='Sandbox_Reserved_301/Gbe1/2'>TextToBeDisplayed</scene>
<Structure load='1m7x' size='500' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />
<Structure load='1m7x' size='500' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />
=Function=
Branching Enzymes contributes to the structure of startch in plants and glycogen in animals and bacteria by catalyzing the formation of a-1,6 brach points in the polysaccharides. The polysaccharide is cleaved at the a-1,4 glucosidic linkage, yidleing a non-reducing end oligosaccharide chain and subsequent attachment to the a-1,6 position. Branching of polysaccharides increased the number of non-reducing ends which makes glycogen more reactive to synthesis and digestion, alos essential for assuring its solubility in the cell.
=Glyogen Storage Disease type IV=
=Glyogen Storage Disease type IV=


Mutations in the gene of Branching Enzyme can result in the accumulation of insoluble glycogen in the cell known as Glycogen Storage Disease type IV (GSD-IV). This results in an impaired enzyme which prevents the formation of branch points in glycogen, producing an insoluble polymer. Different forms of GSD-Iv affects the liver, muscular tissue, and/or the central and peripheral nervous system.  
Mutations in the gene of Branching Enzyme can result in the accumulation of insoluble glycogen in the cell known as Glycogen Storage Disease type IV (GSD-IV). This results in an impaired enzyme which prevents the formation of branch points in glycogen, producing an insoluble polymer. Different forms of GSD-Iv affects the liver, muscular tissue, and/or the central and peripheral nervous system.  
=Family=
=Domains=
=References=
=References=
<references/>
<references/>
[http://www.wikipedia.org E.ColiBranchingEnzyme]
[http://www.wikipedia.org E.ColiBranchingEnzyme]

Revision as of 17:25, 4 April 2011

This Sandbox is Reserved from January 10, 2010, through April 10, 2011 for use in BCMB 307-Proteins course taught by Andrea Gorrell at the University of Northern British Columbia, Prince George, BC, Canada.
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More help: Help:Editing

IntroductionIntroduction

PDB ID 1m7x

Drag the structure with the mouse to rotate
1m7x, resolution 2.30Å ()
Activity: 1,4-alpha-glucan branching enzyme, with EC number 2.4.1.18
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml


[1] [1] Escherichia coli Branching Enzyme (BE) (1,4-a-glucan 6-glucosyltransferase)catalyzes the formation of a-1,6 branch points of glycogen. The enzyme contains three domains: a NH2-terminal seven stranded b-sandwich domain, a COOH-terminal domain, and a central a/b-barrel domain containing the enzyme active site. The branching enzyme belongs to the a-amylase family of enzymes.

Insert caption here

Drag the structure with the mouse to rotate

FunctionFunction

Branching Enzymes contributes to the structure of startch in plants and glycogen in animals and bacteria by catalyzing the formation of a-1,6 brach points in the polysaccharides. The polysaccharide is cleaved at the a-1,4 glucosidic linkage, yidleing a non-reducing end oligosaccharide chain and subsequent attachment to the a-1,6 position. Branching of polysaccharides increased the number of non-reducing ends which makes glycogen more reactive to synthesis and digestion, alos essential for assuring its solubility in the cell.

Glyogen Storage Disease type IVGlyogen Storage Disease type IV

Mutations in the gene of Branching Enzyme can result in the accumulation of insoluble glycogen in the cell known as Glycogen Storage Disease type IV (GSD-IV). This results in an impaired enzyme which prevents the formation of branch points in glycogen, producing an insoluble polymer. Different forms of GSD-Iv affects the liver, muscular tissue, and/or the central and peripheral nervous system.

FamilyFamily

DomainsDomains

ReferencesReferences

  1. ↑ 1.0 1.1 Abad MC, Binderup K, Rios-Steiner J, Arni RK, Preiss J, Geiger JH. The X-ray crystallographic structure of Escherichia coli branching enzyme. J Biol Chem. 2002 Nov 1;277(44):42164-70. Epub 2002 Aug 23. PMID:12196524 doi:10.1074/jbc.M205746200

E.ColiBranchingEnzyme

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA, Alec Lester
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