Sandbox Reserved 327: Difference between revisions
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==eIF1-ribosome binding site== | ==eIF1-ribosome binding site== | ||
eIF1 would bind to the ribosomal 40S, in its P-site region, affecting the conformation of the 40S subunit, the position of mRNA and the start codon initiation <ref name="lomakin"/>. This binding site region is unexpectedly similar to the binding site of prokaryotic initiation factor IF3 to the ribosome, despite their unrelated structure <ref name="lomakin"/>. Together with eIF1A, eIF5, eIF3, and the eIF2.GTP.Met-tRNA<sub>i</sub><sup>Met</sup> ternary complex (TC), eIF1 would form a 43S preinitiation complex (PIC) | eIF1 would bind to the ribosomal 40S, in its P-site region, affecting the conformation of the 40S subunit, the position of mRNA and the start codon initiation <ref name="lomakin"/>. This binding site region is unexpectedly similar to the binding site of prokaryotic initiation factor IF3 to the ribosome, despite their unrelated structure <ref name="lomakin"/>. Together with eIF1A, eIF5, eIF3, and the eIF2.GTP.Met-tRNA<sub>i</sub><sup>Met</sup> ternary complex (TC), eIF1 would form a 43S preinitiation complex (PIC) or also known as multi factor complex (MFC)<ref name="eIF1"/><ref name="lomakin"/><ref name="cheung"/>. | ||
==eIF1-eIF5 binding sites== | ==eIF1-eIF5 binding sites== | ||