Sandbox Reserved 342: Difference between revisions
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Magnesium and other divalent cations are necessary for catalysis because magnesium and PRib-PP binding play a critical role for the PRTase reaction<ref name="Vos"/>. The Mg:PRib-PP complex binds to the active site of PRTases<ref name="Vos"/>. In type I PRTases, XGPRT, catalysis proceeds via SN1 mechanism and it forms a oxocarbonium ion in the transition state<ref name="Vos"/>. It has been suggested, that the magnesium ion departs with the displaced pyrophosphate because there is no magnesium ion at the active site<ref name="Vos"/>. | Magnesium and other divalent cations are necessary for catalysis because magnesium and PRib-PP binding play a critical role for the PRTase reaction<ref name="Vos"/>. The Mg:PRib-PP complex binds to the active site of PRTases<ref name="Vos"/>. In type I PRTases, XGPRT, catalysis proceeds via SN1 mechanism and it forms a oxocarbonium ion in the transition state<ref name="Vos"/>. It has been suggested, that the magnesium ion departs with the displaced pyrophosphate because there is no magnesium ion at the active site<ref name="Vos"/>. | ||
<scene name='Sandbox_Reserved_342/Ligands/1'>Ligands</scene> | <scene name='Sandbox_Reserved_342/Ligands/1'>Ligands</scene> | ||
<ref name="Vos"> RCSB=1a96 </ref> | |||
=References= | =References= | ||
<references/> | <references/> | ||