Sandbox Reserved 327: Difference between revisions
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=Structure and Function= | =Structure and Function= | ||
[[Image:640px-Eukaryotic_initiation.png|thumb|left|Mechanism of Eukaryotic initiation]] | [[Image:640px-Eukaryotic_initiation.png|thumb|left|Mechanism of Eukaryotic initiation]] | ||
==Structure== | |||
eIF1 is one of many eIFs that is needed to make sure the translation initiation process occurs properly <ref name="eIF1"/>. It is a small protein (12 kDa) that would bind to the eIF5 to form multifactor complex and perform AUG selection <ref name="eIF1"/>. | eIF1 is one of many eIFs that is needed to make sure the translation initiation process occurs properly <ref name="eIF1"/>. It is a small protein (12 kDa) that would bind to the eIF5 to form multifactor complex and perform AUG selection <ref name="eIF1"/>. | ||
*possible green link: ribbon structure of eIF1 | *possible green link: ribbon structure of eIF1 | ||
<scene name='Sandbox_Reserved_327/Ribbon_structure/1'>Ribbon Structure</scene> | <scene name='Sandbox_Reserved_327/Ribbon_structure/1'>Ribbon Structure</scene> | ||
*encoded by ''SUI1'' | *encoded by ''SUI1'' | ||
compared to human?? | |||
==Function== | |||
*regulator of start site selection | *regulator of start site selection | ||
*possible figure: show where eIF1 located in the ribosome | *possible figure: show where eIF1 located in the ribosome | ||
==Start Codon Selection | ===Start Codon Selection=== | ||
stimulates the recruitment of TC to the 40s subunit | stimulates the recruitment of TC to the 40s subunit | ||
== | ===Multifactor Complex Assembly=== | ||
* | * | ||
=Mechanism= | =Mechanism= | ||
==binding | overall? | ||
==eIF1-ribosome binding site== | |||
how its bind | how its bind | ||
==binding | ==eIF1-eIF5 binding sites== | ||
*possible green link: show binding region of eIF1 to the eIF5 --> surface representation | *possible green link: show binding region of eIF1 to the eIF5 --> surface representation | ||
<scene name='Sandbox_Reserved_327/Basic_eif1/3'>eIF5 binding site</scene> | <scene name='Sandbox_Reserved_327/Basic_eif1/3'>eIF5 binding site</scene> | ||
*possible figure: assembly with eIF5 | *possible figure: assembly with eIF5 | ||
=how it's released after AUG recognition?= | |||
*possible figure: the release of eIF1 | *possible figure: the release of eIF1 | ||
=Mutations= | =Mutations= | ||
==at the eIF1-eIF5 binding site== | ==at the eIF1-eIF5 binding site== | ||
*altering the basic part of eIF1-KH | *altering the basic part of eIF1-KH | ||
*altering the hydrophobic residues of eIF1 | *altering the hydrophobic residues of eIF1 | ||
==others?== | ==others?== | ||