Sandbox Reserved 200: Difference between revisions

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=Introduction=
=Introduction=
[[Image:2D_RNase_A.png|200px|left|thumb|RNase A minor dimer,[[1A2W]]]]
[[Image:2D_RNase_A.png|200px|left|thumb|RNase A minor dimer,[[1A2W]]]]
Bovine pancreatic ribonuclease A (RNase A) is an enzyme that catalyzes the hydrolysis of RNA through [[Proteopedia:Sandbox_Reserved_193|acid-base catalysis]].  RNase A has the capability to structurally form dimers, trimers, and other oligomers based on the structure of the [[Proteopedia:Sandbox_Reserved_192|monomer]].  The oligomers are formed by 3D domain swapping, which can occur once or twice per monomeric unit <ref name="liul">PMID:11224563</ref >.  The 3D domain swapping has no impact on the formation of active sites.  These same active sites, which are composed of His 12, Lys 41, and His 119, can be found in the monomer, dimer, and trimer <ref name="liul"/>.     
Bovine pancreatic ribonuclease A (RNase A) is an enzyme that catalyzes the hydrolysis of RNA through [[Proteopedia:Sandbox_reserved_193|acid-base catalysis]].  RNase A has the capability to structurally form dimers, trimers, and other oligomers based on the structure of the [[Proteopedia:Sandbox_Reserved_192|monomer]].  The oligomers are formed by 3D domain swapping, which can occur once or twice per monomeric unit <ref name="liul">PMID:11224563</ref >.  The 3D domain swapping has no impact on the formation of active sites.  These same active sites, which are composed of His 12, Lys 41, and His 119, can be found in the monomer, dimer, and trimer <ref name="liul"/>.     
<Structure load='1A2W' size='400' frame='true' align='right' caption='Ribonuclease A Dimer' scene='Sandbox_Reserved_200/Minor_dimer/2' />
<Structure load='1A2W' size='400' frame='true' align='right' caption='Ribonuclease A Dimer' scene='Sandbox_Reserved_200/Minor_dimer/2' />


Revision as of 06:24, 31 March 2011

This Sandbox is Reserved from Feb 02, 2011, through Jul 31, 2011 for use by the Biochemistry II class at the Butler University at Indianapolis, IN USA taught by R. Jeremy Johnson. This reservation includes Sandbox Reserved 191 through Sandbox Reserved 200.
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IntroductionIntroduction

File:2D RNase A.png
RNase A minor dimer,1A2W

Bovine pancreatic ribonuclease A (RNase A) is an enzyme that catalyzes the hydrolysis of RNA through acid-base catalysis. RNase A has the capability to structurally form dimers, trimers, and other oligomers based on the structure of the monomer. The oligomers are formed by 3D domain swapping, which can occur once or twice per monomeric unit [1]. The 3D domain swapping has no impact on the formation of active sites. These same active sites, which are composed of His 12, Lys 41, and His 119, can be found in the monomer, dimer, and trimer [1].

Ribonuclease A Dimer

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Minor DimerMinor Dimer

Major DimerMajor Dimer

TrimersTrimers

Ribonuclease A Minor Trimer

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Medical RelevanceMedical Relevance

The RNase A 3D swapped dimers show significant biological activity. This biological activity includes allostery, antitumor and immunorepression activity. This same activity was not observed in the monomer and the non-3D domain swapped dimers .[2] This could be due to the fact that the monomer has a cystolic RNase A inhibitor. [3]

Literature CitedLiterature Cited

  1. ↑ 1.0 1.1 Liu Y, Gotte G, Libonati M, Eisenberg D. A domain-swapped RNase A dimer with implications for amyloid formation. Nat Struct Biol. 2001 Mar;8(3):211-4. PMID:11224563 doi:10.1038/84941
  2. ↑ Clemo FA. Urinary enzyme evaluation of nephrotoxicity in the dog. Toxicol Pathol. 1998 Jan-Feb;26(1):29-32. PMID:9502384
  3. ↑ Liu Y, Gotte G, Libonati M, Eisenberg D. Structures of the two 3D domain-swapped RNase A trimers. Protein Sci. 2002 Feb;11(2):371-80. PMID:11790847

External LinksExternal Links

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA, Lexi Gehring, R. Jeremy Johnson, Jaime Prilusky
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