Prp24: Difference between revisions
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===RNA Recognition Motifs=== | ===RNA Recognition Motifs=== | ||
Prp24 contains four RRMs, RRM 1, RRM 2, RRM 3, and RRM 4. These motifs have a canonical structure of a platform of four | Prp24 contains four RRMs, RRM 1, RRM 2, RRM 3, and RRM 4. These motifs have a canonical structure of a platform of four β-strands with two α-helices on one side of the &beta sheet plane (green link). These RRMs are present in many proteins that bind to to single stranded regions of RNA (reference!!!!) and their presence in Prp24 supports a role for the annealing of U4 and U6 snRNAs into the U4/U6 structure. | ||
Within each RRM, there are two RNP consensus domains. These are the regions in the &beta*** strands that are thought to actually interact with the RNA. These regions seem to be very important in Prp24 and its interaction with U4 and U6. The study that first identified a probable link between the Prp24 protein and U4/U6 found that mutations in RNP 1 and RNP 2 of the carboxy terminal RRM (green link) rescued a cold-sensitive phenotype caused by a U4 mutation in stem II of U4/U6 (Shannon and Guthrie 1991). Two further studies (reference Vidaver et al. 1999 and Kwan and Brow 2005) showed that the mutation of three highly conserved residues in the RNP domains of any of the four RRMs (green link) conferred either temperature-sensitive growth or lethality to yeast cells. | Within each RRM, there are two RNP consensus domains. These are the regions in the &beta*** strands that are thought to actually interact with the RNA. These regions seem to be very important in Prp24 and its interaction with U4 and U6. The study that first identified a probable link between the Prp24 protein and U4/U6 found that mutations in RNP 1 and RNP 2 of the carboxy terminal RRM (green link) rescued a cold-sensitive phenotype caused by a U4 mutation in stem II of U4/U6 (Shannon and Guthrie 1991). Two further studies (reference Vidaver et al. 1999 and Kwan and Brow 2005) showed that the mutation of three highly conserved residues in the RNP domains of any of the four RRMs (green link) conferred either temperature-sensitive growth or lethality to yeast cells. | ||