Sandbox Reserved 327: Difference between revisions
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Yeast eIF1 | Yeast eIF1 | ||
=Introduction= | =Introduction= | ||
Translation is an essential process for both prokaryotes and eukaryotes to make various proteins from nucleic acid. It requires three different steps: initiation, elongation, and termination. Many proteins are needed in the initiation phase to form an initiation complex with the ribosome and promote the translation. In eukaryotes, these proteins are also known as eukaryotic initiation factors (eIF). The eIFs were first studied through the genetic analyzed of yeast ''Saccharomyces cerevisiae'' <ref name="elegans"/>. The initiation process is complex since it involves at least 12 eIFs containing more than 30 polypeptides , including eIF1, eIF2, eIF3, eIF4, and eIF5 <ref name="elegans"/>. | Translation is an essential process for both prokaryotes and eukaryotes to make various proteins from nucleic acid. It requires three different steps: initiation, elongation, and termination. Many proteins are needed in the initiation phase to form an initiation complex with the ribosome and promote the translation. In eukaryotes, these proteins are also known as eukaryotic initiation factors (eIF). The eIFs were first studied through the genetic analyzed of yeast ''Saccharomyces cerevisiae'' <ref name="elegans"/>. The initiation process is complex since it involves at least 12 eIFs containing more than 30 polypeptides , including eIF1, eIF2, eIF3, eIF4, and eIF5 <ref name="elegans"/>. | ||
{{STRUCTURE_2ogh|PDB=2ogh|SCENE}} | {{STRUCTURE_2ogh|PDB=2ogh|SCENE}} | ||
=Structure and Function= | =Structure and Function= | ||
[[Image:640px-Eukaryotic_initiation.png|thumb|left|Mechanism of Eukaryotic initiation]] | |||
eIF1 is one of many eIFs that is needed to make sure the translation initiation process occurs properly <ref name="eIF1"/>. It is a small protein (12 kDa) that would bind to the eIF5 to form multifactor complex and perform AUG selection <ref name="eIF1"/>. | eIF1 is one of many eIFs that is needed to make sure the translation initiation process occurs properly <ref name="eIF1"/>. It is a small protein (12 kDa) that would bind to the eIF5 to form multifactor complex and perform AUG selection <ref name="eIF1"/>. | ||
*possible green link: ribbon structure of eIF1 | *possible green link: ribbon structure of eIF1 | ||
<scene name='Sandbox_Reserved_327/Ribbon_structure/1'>Ribbon Structure</scene> | <scene name='Sandbox_Reserved_327/Ribbon_structure/1'>Ribbon Structure</scene> | ||
* | *encoded by ''SUI1'' | ||
*possible figure: show where eIF1 located in the ribosome | *possible figure: show where eIF1 located in the ribosome | ||