2oan: Difference between revisions
New page: left|200px<br /><applet load="2oan" size="450" color="white" frame="true" align="right" spinBox="true" caption="2oan, resolution 2.606Å" /> '''Structure of oxidiz... |
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[[Image:2oan.jpg|left|200px]]<br /><applet load="2oan" size=" | [[Image:2oan.jpg|left|200px]]<br /><applet load="2oan" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2oan, resolution 2.606Å" /> | caption="2oan, resolution 2.606Å" /> | ||
'''Structure of oxidized beta-actin'''<br /> | '''Structure of oxidized beta-actin'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2OAN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with CA, SO4, OC4 and ATP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2OAN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=OC4:'>OC4</scene> and <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OAN OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: disulfide]] | [[Category: disulfide]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:53:21 2008'' | ||
Revision as of 15:53, 23 January 2008
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Structure of oxidized beta-actin
OverviewOverview
An essential consequence of growth factor-mediated signal transduction is, the generation of intracellular H(2)O(2). It operates as a second, messenger in the control of actin microfilament dynamics, causing rapid, and dramatic changes in the morphology and motile activity of stimulated, cells. Little is understood about the molecular mechanisms causing these, changes in the actin system. Here, it is shown that H(2)O(2) acts directly, upon several levels of this system, and some of the mechanistic effects, are detailed. We describe the impact of oxidation on the polymerizability, of non-muscle beta/gamma-actin and compare with that of muscle, alpha-actin. Oxidation of beta/gamma-actin can cause a complete loss of, polymerizability, crucially, reversible by the thioredoxin system., Further, oxidation of the actin impedes its interaction with profilin and, causes depolymerization of filamentous actin. The effects of oxidation are, critically dependent on the nucleotide state and the concentration of, Ca(2+). We have determined the crystal structure of oxidized beta-actin to, a resolution of 2.6 A. The arrangement in the crystal implies an, antiparallel homodimer connected by an intermolecular disulfide bond, involving cysteine 374. Our data indicate that this dimer forms under, non-polymerizing and oxidizing conditions. We identify oxidation of, cysteine 272 in the crystallized actin dimer, likely to a cysteine, sulfinic acid. In beta/gamma-actin, this is the cysteine residue most, reactive towards H(2)O(2) in solution, and we suggest plausible structural, determinants for its reactivity. No other oxidative modification was, obvious in the structure, highlighting the specificity of the oxidation by, H(2)O(2). Possible consequences of the observed effects in a cellular, context and their potential relevance are discussed.
About this StructureAbout this Structure
2OAN is a Single protein structure of sequence from Bos taurus with , , and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Molecular and Structural Basis for Redox Regulation of beta-Actin., Lassing I, Schmitzberger F, Bjornstedt M, Holmgren A, Nordlund P, Schutt CE, Lindberg U, J Mol Biol. 2007 Jul 6;370(2):331-48. Epub 2007 May 4. PMID:17521670
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