2gqe: Difference between revisions
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'''Molecular characterization of the Ran binding zinc finger domain'''<br /> | '''Molecular characterization of the Ran binding zinc finger domain'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2GQE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 2GQE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GQE OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: zinc knuckle]] | [[Category: zinc knuckle]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:52:44 2008'' | ||
Revision as of 15:52, 23 January 2008
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Molecular characterization of the Ran binding zinc finger domain
OverviewOverview
The nuclear pore complex is the gateway for selective traffic between the, nucleus and cytoplasm. To learn how building blocks of the pore can create, specific docking sites for transport receptors and regulatory factors, we, have studied a zinc finger module present in multiple copies within the, nuclear pores of higher eukaryotes. All four zinc fingers of human Nup153, were found to bind the small GTPase Ran with dissociation constants, ranging between 5 and 40 mum. In addition a fragment of Nup153, encompassing the four tandem zinc fingers was found to bind Ran with, similar affinity. NMR structural studies revealed that a representative, Nup153 zinc finger adopts the same zinc ribbon structure as the previously, characterized Npl4 NZF module. Ran binding was mediated by a three-amino, acid motif (Leu(13)/Val(14)/Asn(25)) located within the two zinc, coordination loops. Nup153 ZnFs bound GDP and GTP forms of Ran with, similar affinities, indicating that this interaction is not influenced by, a nucleotide-dependent conformational switch. Taken together, these, studies elucidate the Ran-binding interface on Nup153 and, more broadly, provide insight into the versatility of this zinc finger binding module.
About this StructureAbout this Structure
2GQE is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Molecular Characterization of the Ran-binding Zinc Finger Domain of Nup153., Higa MM, Alam SL, Sundquist WI, Ullman KS, J Biol Chem. 2007 Jun 8;282(23):17090-100. Epub 2007 Apr 10. PMID:17426026
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