2c7a: Difference between revisions
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[[Image:2c7a.gif|left|200px]]<br /><applet load="2c7a" size=" | [[Image:2c7a.gif|left|200px]]<br /><applet load="2c7a" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2c7a, resolution 2.50Å" /> | caption="2c7a, resolution 2.50Å" /> | ||
'''STRUCTURE OF THE PROGESTERONE RECEPTOR-DNA COMPLEX'''<br /> | '''STRUCTURE OF THE PROGESTERONE RECEPTOR-DNA COMPLEX'''<br /> | ||
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==Overview== | ==Overview== | ||
The DNA binding domain (DBD) of nuclear hormone receptors contains a, highly conserved globular domain and a less conserved carboxyl-terminal, extension (CTE). Despite previous observations that the CTEs of some, classes of nuclear receptors are structured and interact with DNA outside, of the hexanucleotide hormone response element (HRE), there has been no, evidence for such a CTE among the steroid receptors. We have determined, the structure of the progesterone receptor (PR)-DBD-CTE DNA complex at a, resolution of 2.5 A, which revealed binding of the CTE to the minor groove, flanking the HREs. Alanine substitutions of the interacting CTE residues, reduced affinity for inverted repeat HREs separated by three nucleotides, and essentially abrogated binding to a single HRE. A highly compressed, minor groove of the trinucleotide spacer and a novel dimerization, interface were also observed. A PR binding site selection experiment, revealed sequence preferences in the trinucleotide spacer and flanking, DNA. These results, taken together, support the notion that sequences, outside of the HREs influence the DNA binding affinity and specificity of, steroid receptors. | The DNA binding domain (DBD) of nuclear hormone receptors contains a, highly conserved globular domain and a less conserved carboxyl-terminal, extension (CTE). Despite previous observations that the CTEs of some, classes of nuclear receptors are structured and interact with DNA outside, of the hexanucleotide hormone response element (HRE), there has been no, evidence for such a CTE among the steroid receptors. We have determined, the structure of the progesterone receptor (PR)-DBD-CTE DNA complex at a, resolution of 2.5 A, which revealed binding of the CTE to the minor groove, flanking the HREs. Alanine substitutions of the interacting CTE residues, reduced affinity for inverted repeat HREs separated by three nucleotides, and essentially abrogated binding to a single HRE. A highly compressed, minor groove of the trinucleotide spacer and a novel dimerization, interface were also observed. A PR binding site selection experiment, revealed sequence preferences in the trinucleotide spacer and flanking, DNA. These results, taken together, support the notion that sequences, outside of the HREs influence the DNA binding affinity and specificity of, steroid receptors. | ||
==About this Structure== | ==About this Structure== | ||
2C7A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Zn Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | 2C7A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Zn Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7A OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:52:33 2008'' | ||
Revision as of 15:52, 23 January 2008
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STRUCTURE OF THE PROGESTERONE RECEPTOR-DNA COMPLEX
OverviewOverview
The DNA binding domain (DBD) of nuclear hormone receptors contains a, highly conserved globular domain and a less conserved carboxyl-terminal, extension (CTE). Despite previous observations that the CTEs of some, classes of nuclear receptors are structured and interact with DNA outside, of the hexanucleotide hormone response element (HRE), there has been no, evidence for such a CTE among the steroid receptors. We have determined, the structure of the progesterone receptor (PR)-DBD-CTE DNA complex at a, resolution of 2.5 A, which revealed binding of the CTE to the minor groove, flanking the HREs. Alanine substitutions of the interacting CTE residues, reduced affinity for inverted repeat HREs separated by three nucleotides, and essentially abrogated binding to a single HRE. A highly compressed, minor groove of the trinucleotide spacer and a novel dimerization, interface were also observed. A PR binding site selection experiment, revealed sequence preferences in the trinucleotide spacer and flanking, DNA. These results, taken together, support the notion that sequences, outside of the HREs influence the DNA binding affinity and specificity of, steroid receptors.
About this StructureAbout this Structure
2C7A is a Single protein structure of sequence from Homo sapiens with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Structure of the progesterone receptor-deoxyribonucleic acid complex: novel interactions required for binding to half-site response elements., Roemer SC, Donham DC, Sherman L, Pon VH, Edwards DP, Churchill ME, Mol Endocrinol. 2006 Dec;20(12):3042-52. Epub 2006 Aug 24. PMID:16931575
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