2e51: Difference between revisions
New page: left|200px<br /><applet load="2e51" size="450" color="white" frame="true" align="right" spinBox="true" caption="2e51, resolution 2.50Å" /> '''Crystal structure of... |
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[[Image:2e51.gif|left|200px]]<br /><applet load="2e51" size=" | [[Image:2e51.gif|left|200px]]<br /><applet load="2e51" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2e51, resolution 2.50Å" /> | caption="2e51, resolution 2.50Å" /> | ||
'''Crystal structure of basic winged bean lectin in complex with A blood group disaccharide'''<br /> | '''Crystal structure of basic winged bean lectin in complex with A blood group disaccharide'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2E51 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus] with MN and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2E51 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Psophocarpus_tetragonolobus Psophocarpus tetragonolobus] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E51 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:44:10 2008'' | ||
Revision as of 15:44, 23 January 2008
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Crystal structure of basic winged bean lectin in complex with A blood group disaccharide
OverviewOverview
Basic winged bean agglutinin binds A-blood group substance with higher, affinity and B-blood group substance with lesser affinity. It does not, bind the O substance. The crystal structures of the lectin, complexed with, A-reactive and B-reactive di and tri saccharides, have been determined. In, addition, the complexes of the lectin with fucosylated A-trisaccharides, and B-trisaccharides and with a variant of the A-trisaccharide have been, modeled. These structures and models provide valuable insights into the, structural basis of blood group specificities. All the four carbohydrate, binding loops of the lectin contribute to the primary combining site while, the loop of variable length contributes to the secondary binding site. In, a significant advance to the current understanding, the interactions at, the secondary binding site also contribute substantially, albeit in a, subtle manner, to determine the blood group specificity. Compared with the, interactions of the B-trisaccharide with the lectin, the third sugar, residue of the A-reactive trisacharide forms an additional hydrogen bond, with a lysine residue in the variable loop. In the former, the formation, of such a hydrogen bond is prevented by a shift in the orientation of, third sugar resulting from an internal hydrogen bond in it. The formation, of this bond is also facilitated by an interaction dependent change in the, rotamer conformation of the lysyl residue of the variable loop. Thus, the, difference in the interactions at the secondary site is generated by, coordinated movements in the ligand as well as the protein. A comparison, of the crystal structure and the model of the complex involving the, variant of the A-trisaccharide results in the delineation of the relative, contributions of the interactions at the primary and the secondary sites, in determining blood group specificity. Proteins 2007. (c) 2007, Wiley-Liss, Inc.
About this StructureAbout this Structure
2E51 is a Single protein structure of sequence from Psophocarpus tetragonolobus with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Generation of blood group specificity: New insights from structural studies on the complexes of A- and B-reactive saccharides with basic winged bean agglutinin., Kulkarni KA, Katiyar S, Surolia A, Vijayan M, Suguna K, Proteins. 2007 May 17;. PMID:17510954
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