1wq2: Difference between revisions
New page: left|200px<br /><applet load="1wq2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wq2, resolution 2.4Å" /> '''Neutron Crystal Struc... |
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[[Image:1wq2.gif|left|200px]]<br /><applet load="1wq2" size=" | [[Image:1wq2.gif|left|200px]]<br /><applet load="1wq2" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="1wq2, resolution 2.4Å" /> | caption="1wq2, resolution 2.4Å" /> | ||
'''Neutron Crystal Structure Of Dissimilatory Sulfite Reductase D (DsrD)'''<br /> | '''Neutron Crystal Structure Of Dissimilatory Sulfite Reductase D (DsrD)'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
1WQ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with SO4 and DOD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 1WQ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=DOD:'>DOD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WQ2 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: neutron hydrogen hydration protein]] | [[Category: neutron hydrogen hydration protein]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:42:16 2008'' | ||
Revision as of 15:42, 23 January 2008
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Neutron Crystal Structure Of Dissimilatory Sulfite Reductase D (DsrD)
OverviewOverview
Dissimilatory sulfite reductase D (DsrD) from Desulfovibrio vulgaris has, been crystallized for a neutron diffraction study. The initial crystals, obtained were too small for the neutron experiment. In order to obtain a, larger crystal (>1 mm3), a combination of two techniques was developed to, determine the optimum crystallization conditions: a crystallization phase, diagram was obtained, followed by crystal-quality assessment via X-ray, diffraction. Using conditions determined in this manner, a large single, crystal (1.7 mm3) of DsrD protein was subsequently grown in D(2)O solution, by the macroseeding technique. A neutron diffraction experiment was, carried out using the BIX-3 diffractometer at the Japan Atomic Energy, Research Institute (JAERI), collecting data to 2.4 A resolution from an, optimized crystal.
About this StructureAbout this Structure
1WQ2 is a Single protein structure of sequence from Desulfovibrio vulgaris with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Crystallization and preliminary neutron analysis of the dissimilatory sulfite reductase D (DsrD) protein from the sulfate-reducing bacterium Desulfovibrio vulgaris., Chatake T, Mizuno N, Voordouw G, Higuchi Y, Arai S, Tanaka I, Niimura N, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2306-9. Epub 2003, Nov 27. PMID:14646103
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