1a40: Difference between revisions
New page: left|200px<br /> <applet load="1a40" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a40, resolution 1.70Å" /> '''PHOSPHATE-BINDING P... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
1A40 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with PO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A40 OCA]]. | 1A40 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with PO4 as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: BNG. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A40 OCA]]. | ||
==Reference== | ==Reference== | ||
| Line 25: | Line 25: | ||
[[Category: phosphate-binding protein]] | [[Category: phosphate-binding protein]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:44:48 2007'' | ||
Revision as of 12:40, 30 October 2007
|
PHOSPHATE-BINDING PROTEIN WITH ALA 197 REPLACED WITH TRP
OverviewOverview
Stringent specificity and complementarity between the receptor, a, periplasmic phosphate-binding protein (PBP) with a two-domain structure, and the completely buried and dehydrated phosphate are achieved by, hydrogen bonding or dipolar interactions. We recently found that the, surface charge potential of the cleft between the two domains that, contains the anion binding site is intensely electronegative. This novel, finding prompted the study reported here of the effect of ionic strength, on the equilibrium and rapid kinetics of phosphate binding. To facilitate, this study, Ala197, located on the edge of the cleft, was replaced by a, Trp residue (A197W PBP) to generate a fluorescence reporter group. The, A197W PBP-phosphate complex retains wild-type Kd and X-ray structure, beyond the ... [(full description)]
About this StructureAbout this Structure
1A40 is a [Single protein] structure of sequence from [Escherichia coli] with PO4 as [ligand]. Structure known Active Site: BNG. Full crystallographic information is available from [OCA].
ReferenceReference
Dominant role of local dipolar interactions in phosphate binding to a receptor cleft with an electronegative charge surface: equilibrium, kinetic, and crystallographic studies., Ledvina PS, Tsai AL, Wang Z, Koehl E, Quiocho FA, Protein Sci. 1998 Dec;7(12):2550-9. PMID:9865949
Page seeded by OCA on Tue Oct 30 11:44:48 2007