2o6g: Difference between revisions
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'''Crystal structure of IRF-3 bound to the interferon-b enhancer'''<br /> | '''Crystal structure of IRF-3 bound to the interferon-b enhancer'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2O6G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | 2O6G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O6G OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:25:22 2008'' | ||
Revision as of 15:25, 23 January 2008
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Crystal structure of IRF-3 bound to the interferon-b enhancer
OverviewOverview
Transcriptional activation of the interferon-beta (IFN-beta) gene requires, assembly of an enhanceosome containing ATF-2/c-Jun, IRF-3/IRF-7, and, NFkappaB. These factors bind cooperatively to the IFN-beta enhancer and, recruit coactivators and chromatin-remodeling proteins to the IFN-beta, promoter. We describe here a crystal structure of the DNA-binding domains, of IRF-3, IRF-7, and NFkappaB, bound to one half of the enhancer, and use, a previously described structure of the remaining half to assemble a, complete picture of enhanceosome architecture in the vicinity of the DNA., Association of eight proteins with the enhancer creates a continuous, surface for recognizing a composite DNA-binding element. Paucity of local, protein-protein contacts suggests that cooperative occupancy of the, enhancer comes from both binding-induced changes in DNA conformation and, interactions with additional components such as CBP. Contacts with, virtually every nucleotide pair account for the evolutionary invariance of, the enhancer sequence.
About this StructureAbout this Structure
2O6G is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
An Atomic Model of the Interferon-beta Enhanceosome., Panne D, Maniatis T, Harrison SC, Cell. 2007 Jun 15;129(6):1111-23. PMID:17574024
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