2px2: Difference between revisions

Revision as of 15:23, 23 January 2008

Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Monoclinic form 1)

OverviewOverview

We have determined the high resolution crystal structure of the, methyltransferase domain of the NS5 polypeptide from the Murray Valley, encephalitis virus. This domain is unusual in having both the N7 and 2'-O, methyltransferase activity required for Cap 1 synthesis. We have also, determined structures for complexes of this domain with nucleotides and, cap analogues providing information on cap binding, based on which we, suggest a model of how the sequential methylation of the N7 and 2'-O, groups of the cap may be coordinated.

About this StructureAbout this Structure

2PX2 is a Single protein structure of sequence from Murray valley encephalitis virus with and as ligands. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the Murray Valley encephalitis virus NS5 methyltransferase domain in complex with cap analogues., Assenberg R, Ren J, Verma A, Walter TS, Alderton D, Hurrelbrink RJ, Fuller SD, Bressanelli S, Owens RJ, Stuart DI, Grimes JM, J Gen Virol. 2007 Aug;88(Pt 8):2228-36. PMID:17622627

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OCA

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-[[Image:2px2.jpg|left|200px]]<br /><applet load="2px2" size="450" color="white" frame="true" align="right" spinBox="true"
+[[Image:2px2.jpg|left|200px]]<br /><applet load="2px2" size="350" color="white" frame="true" align="right" spinBox="true"
 caption="2px2, resolution 2.00&Aring;" />
 '''Crystal structure of the Murray Valley Encephalitis Virus NS5 2'-O Methyltransferase domain in complex with SAH (Monoclinic form 1)'''<br />
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 ==About this Structure==
-PX2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Murray_valley_encephalitis_virus Murray valley encephalitis virus] with CL and SAH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2PX2 OCA].
+PX2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Murray_valley_encephalitis_virus Murray valley encephalitis virus] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX2 OCA].
 ==Reference==
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 [[Category: structural genomics]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:42:47 2007''
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