2i05: Difference between revisions
New page: left|200px<br /><applet load="2i05" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i05, resolution 2.60Å" /> '''Escherichia Coli Rep... |
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[[Image:2i05.gif|left|200px]]<br /><applet load="2i05" size=" | [[Image:2i05.gif|left|200px]]<br /><applet load="2i05" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2i05, resolution 2.60Å" /> | caption="2i05, resolution 2.60Å" /> | ||
'''Escherichia Coli Replication Terminator Protein (Tus) Complexed With TerA DNA'''<br /> | '''Escherichia Coli Replication Terminator Protein (Tus) Complexed With TerA DNA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2I05 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with IOD as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 2I05 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=IOD:'>IOD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I05 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: replication/dna complex]] | [[Category: replication/dna complex]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 14:00:00 2008'' | ||
Revision as of 15:00, 23 January 2008
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Escherichia Coli Replication Terminator Protein (Tus) Complexed With TerA DNA
OverviewOverview
During chromosome synthesis in Escherichia coli, replication forks are, blocked by Tus bound Ter sites on approach from one direction but not the, other. To study the basis of this polarity, we measured the rates of, dissociation of Tus from forked TerB oligonucleotides, such as would be, produced by the replicative DnaB helicase at both the fork-blocking, (nonpermissive) and permissive ends of the Ter site. Strand separation of, a few nucleotides at the permissive end was sufficient to force rapid, dissociation of Tus to allow fork progression. In contrast, strand, separation extending to and including the strictly conserved G-C(6) base, pair at the nonpermissive end led to formation of a stable locked complex., Lock formation specifically requires the cytosine residue, C(6). The, crystal structure of the locked complex showed that C(6) moves 14 A from, its normal position to bind in a cytosine-specific pocket on the surface, of Tus.
About this StructureAbout this Structure
2I05 is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
A molecular mousetrap determines polarity of termination of DNA replication in E. coli., Mulcair MD, Schaeffer PM, Oakley AJ, Cross HF, Neylon C, Hill TM, Dixon NE, Cell. 2006 Jun 30;125(7):1309-19. PMID:16814717
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