Colicin E3: Difference between revisions
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{{STRUCTURE_2xfz | PDB=2xfz | SCENE= }} | {{STRUCTURE_2xfz | PDB=2xfz | SCENE= }} | ||
The C terminal domain of colicin E3 kills the cells that it penetrates by actively degrading the 16s rRNA subunit of the cell's 70S ribosome. This prevents the cell from producing any proteins once attacked, which will lead to the death of the cell. This rRNA cleavage is specific ''in vivo'', but random when the rRNA domain is isolated ''in vitro''<ref> PMID: 368047 </ref>. The cleavage is unaffected by the presence of cellular ribonucleases<ref> PMID: 4567332 </ref>. The specific cleavage observed ''in vivo'' occurs within the ribosomal decoding A-site, in between A1493 and G1494, resulting in the complete halt of protein biosynthesis, leading to cell death<ref> PMID: 12423788 </ref>. | The C terminal domain of colicin E3 kills the cells that it penetrates with its [[16s rRNase activity]], by actively degrading the 16s rRNA subunit of the cell's 70S ribosome. This prevents the cell from producing any proteins once attacked, which will lead to the death of the cell. This rRNA cleavage is specific ''in vivo'', but random when the rRNA domain is isolated ''in vitro''<ref> PMID: 368047 </ref>. The cleavage is unaffected by the presence of cellular ribonucleases<ref> PMID: 4567332 </ref>. The specific cleavage observed ''in vivo'' occurs within the ribosomal decoding A-site, in between A1493 and G1494, resulting in the complete halt of protein biosynthesis, leading to cell death<ref> PMID: 12423788 </ref>. | ||
==References== | ==References== | ||
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