Colicin E5: Difference between revisions

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 ==Killing Activities==
+{{STRUCTURE_2djh |  PDB=2djh  |  SCENE=  }}
+ColE5 specifically cleaves the anticodons of the tRNAs for tyr, his, asn and asp, which each share the unique sequence UQU in the loop. This activity occurs in the 115 residue C terminal domain, and the loop is cleaved between the Q (modified G) and the 3' U, which corresponds to the first and second letters of the anticodon triplet. This specificity is determined by a code YGUN surrounding the anticodon. It also cleaves the same tRNAs as above when they lack the Q<ref> PMID: 10092236 </ref>, so the base modification unique to ColE5 sensitive tRNAs does not appear to be recognised by ColE5<ref> PMID: 16963495 </ref>.
-ColE5 specifically cleaves the anticodons of the tRNAs for tyr, his, asn and asp, which each share the unique sequence UQU in the loop. This activity occurs in the 115 residue C terminal domain, and the loop is cleaved between the Q (modified G) and the 3' U, which corresponds to the first and second letters of the anticodon triplet. This specificity is determined by a code YGUN surrounding the anticodon. It also cleaves the same tRNAs as above when they lack the Q<ref> PMID: 10092236 </ref>, so the base modification unique to ColE5 sensitive tRNAs does not appear to be recognised by ColE5<ref> PMID: 16963495 </ref>.
+The structure shows the crystal structure of the ribonuclease domain of ColE5.