Colicin E9: Difference between revisions
New page: Colicin E9 is a type of Colicin, a bacteriocin made by "E. Coli" which acts against other nearby "E. Coli" to kill them with its DNase activity; it digests the host's genome at specifi... |
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Colicin E9 is a type of [[Colicin]], a bacteriocin made by | Colicin E9 is a type of [[Colicin]], a bacteriocin made by ''E. Coli'' which acts against other nearby ''E. Coli'' to kill them with its DNase activity; it digests the host's genome at specific locations, ultimately leading to the death of the cell. | ||
==Synthesis and release== | ==Synthesis and release== | ||
==Mechanism of uptake== | ==Mechanism of uptake== | ||
The primary receptor for colicin E9 is the vitamin B12 receptor, BtuB. It then requires the outer membrane porin OmpF - either the two form the functional receptor, or OmpF is recruited for subsequent translocation. The OmpF association with the BtuB-colicin complex is weak and transient. After the interaction with OmpF, colicin E9 requires the [[Tol]] system to pass across the periplasm. | |||
OmpF acts synergistically with BtuB to protect bacteria against the action of colicin E9. This could indicate that OmpF is a component of the receptor apparatus. Alternatively the role of OmpF could be more to do with translocation rather than receptor recognition. | |||
<ref> PMID: 12804762 </ref> | |||
==Killing Activities== | ==Killing Activities== | ||
The cytotoxic activity of colE9 is DNase activity. <ref> PMID: 12804762 </ref> | |||
The endonuclease activity of colicin E9 forms channels in planar lipid bilayers. The E9 DNase mediates its own translocation across the cytoplasmic membrane, and the formation of ion channels is essential to this process. <ref> PMID: 15044477 <ref/> | |||
==References== | |||
<references/> | |||