3eq3: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
[[3eq3]] is a 9 chain structure of [[Ribosomal protein S12]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EQ3 OCA]. | |||
==See Also== | |||
*[[Ribosomal protein S12]] | |||
==Reference== | ==Reference== | ||
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[[Category: Ternary complex]] | [[Category: Ternary complex]] | ||
[[Category: Trna-binding]] | [[Category: Trna-binding]] | ||
Revision as of 00:37, 27 December 2010
Model of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EMModel of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM
The accuracy of ribosomal translation is achieved by an initial selection and a proofreading step, mediated by EF-Tu, which forms a ternary complex with aminoacyl(aa)-tRNA. To study the binding modes of different aa-tRNAs, we compared cryo-EM maps of the kirromycin-stalled ribosome bound with ternary complexes containing Phe-tRNA(Phe), Trp-tRNA(Trp), or Leu-tRNA(LeuI). The three maps suggest a common binding manner of cognate aa-tRNAs in their specific binding with both the ribosome and EF-Tu. All three aa-tRNAs have the same 'loaded spring' conformation with a kink and twist between the D-stem and anticodon stem. The three complexes are similarly integrated in an interaction network, extending from the anticodon loop through h44 and protein S12 to the EF-Tu-binding CCA end of aa-tRNA, proposed to signal cognate codon-anticodon interaction to the GTPase centre and tune the accuracy of aa-tRNA selection.
Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM., Li W, Agirrezabala X, Lei J, Bouakaz L, Brunelle JL, Ortiz-Meoz RF, Green R, Sanyal S, Ehrenberg M, Frank J, EMBO J. 2008 Dec 17;27(24):3322-31. Epub 2008 Nov 20. PMID:19020518
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this StructureAbout this Structure
3eq3 is a 9 chain structure of Ribosomal protein S12 with sequence from Escherichia coli k12. Full crystallographic information is available from OCA.
See AlsoSee Also
ReferenceReference
- ↑ Li W, Agirrezabala X, Lei J, Bouakaz L, Brunelle JL, Ortiz-Meoz RF, Green R, Sanyal S, Ehrenberg M, Frank J. Recognition of aminoacyl-tRNA: a common molecular mechanism revealed by cryo-EM. EMBO J. 2008 Dec 17;27(24):3322-31. Epub 2008 Nov 20. PMID:19020518 doi:10.1038/emboj.2008.243
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OCA- Pages with broken file links
- Escherichia coli k12
- Agirrezabala, X.
- Frank, J.
- Li, W.
- A/t-trna
- Acetylation
- Antibiotic resistance
- Automated data collection
- Cytoplasm
- Elongation factor
- Gtp-binding
- Membrane
- Methylation
- Nucleotide-binding
- Phosphoprotein
- Protein biosynthesis
- Protein translation
- Ribonucleoprotein
- Ribosomal protein
- Ribosomal protein/rna complex
- Rna-binding
- Rrna-binding
- Ternary complex
- Trna-binding