2iry: Difference between revisions
New page: left|200px<br /><applet load="2iry" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iry, resolution 1.78Å" /> '''Crystal Structure of... |
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[[Image:2iry.gif|left|200px]]<br /><applet load="2iry" size=" | [[Image:2iry.gif|left|200px]]<br /><applet load="2iry" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2iry, resolution 1.78Å" /> | caption="2iry, resolution 1.78Å" /> | ||
'''Crystal Structure of the Polymerase Domain from Mycobacterium tuberculosis Ligase D with dGTP and Manganese.'''<br /> | '''Crystal Structure of the Polymerase Domain from Mycobacterium tuberculosis Ligase D with dGTP and Manganese.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2IRY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with MN and DGT as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | 2IRY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=DGT:'>DGT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IRY OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:32:05 2008'' | ||
Revision as of 13:32, 23 January 2008
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Crystal Structure of the Polymerase Domain from Mycobacterium tuberculosis Ligase D with dGTP and Manganese.
OverviewOverview
Non homologous end-joining (NHEJ)-mediated repair of DNA double-strand, breaks in prokaryotes requires Ku and a specific multidomain DNA ligase, (LigD). We present crystal structures of the primase/polymerisation domain, (PolDom) of Mycobacterium tuberculosis LigD, alone and complexed with, nucleotides. The PolDom structure combines the general fold of the, archaeo-eukaryotic primase (AEP) superfamily with additional loops and, domains that together form a deep cleft on the surface, likely used for, DNA binding. Enzymatic analysis indicates that the PolDom of LigD, even in, the absence of accessory domains and Ku proteins, has the potential to, recognise DNA end-joining intermediates. Strikingly, one of the main, signals for the specific and efficient binding of PolDom to DNA is the, presence of a 5'-phosphate group, located at the single/double-stranded, junction at both gapped and 3'-protruding DNA molecules. Although, structurally unrelated, Pol lambda and Pol mu, the two eukaryotic DNA, polymerases involved in NHEJ, are endowed with a similar capacity to bind, a 5'-phosphate group. Other properties that are beneficial for NHEJ, such, as the ability to generate template distortions and realignments of the, primer, displayed by Pol lambda and Pol mu, are shared by the PolDom of, bacterial LigD. In addition, PolDom can perform non-mutagenic translesion, synthesis on termini containing modified bases. Significantly, ribonucleotide insertion appears to be a recurrent theme associated with, NHEJ, maximised in this case by the deployment of a dedicated primase, although its in vivo relevance is unknown.
About this StructureAbout this Structure
2IRY is a Single protein structure of sequence from Mycobacterium tuberculosis with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Structure and function of a mycobacterial NHEJ DNA repair polymerase., Pitcher RS, Brissett NC, Picher AJ, Andrade P, Juarez R, Thompson D, Fox GC, Blanco L, Doherty AJ, J Mol Biol. 2007 Feb 16;366(2):391-405. Epub 2006 Oct 20. PMID:17174332
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