2gtv: Difference between revisions
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'''NMR structure of monomeric chorismate mutase from Methanococcus jannaschii'''<br /> | '''NMR structure of monomeric chorismate mutase from Methanococcus jannaschii'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2GTV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with TSA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Chorismate_mutase Chorismate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.5 5.4.99.5] Full crystallographic information is available from [http:// | 2GTV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=TSA:'>TSA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Chorismate_mutase Chorismate mutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.99.5 5.4.99.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GTV OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 12:02:09 2008'' | ||
Revision as of 13:02, 23 January 2008
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NMR structure of monomeric chorismate mutase from Methanococcus jannaschii
OverviewOverview
Although protein dynamics has been recognized as a potentially important, contributor to enzyme catalysis, structural disorder is generally, considered to reduce catalytic efficiency. This widely held assumption has, recently been challenged by the finding that an engineered chorismate, mutase combines high catalytic activity with the properties of a molten, globule, a loosely packed and highly dynamic conformational ensemble., Taking advantage of the ordering observed upon ligand binding, we have now, used NMR spectroscopy to characterize this enzyme in complex with a, transition-state analog. The complex adopts a helix-bundle structure, as, designed, but retains unprecedented flexibility on the millisecond, timescale across its entire length. Moreover, pre-steady-state kinetics, data show that binding occurs by an induced-fit mechanism on the same, timescale as the enzymatic reaction, linking global conformational, plasticity with efficient catalysis.
About this StructureAbout this Structure
2GTV is a Single protein structure of sequence from Methanocaldococcus jannaschii with as ligand. Active as Chorismate mutase, with EC number 5.4.99.5 Full crystallographic information is available from OCA.
ReferenceReference
Structure and dynamics of a molten globular enzyme., Pervushin K, Vamvaca K, Vogeli B, Hilvert D, Nat Struct Mol Biol. 2007 Nov 11;. PMID:17994104
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