2pi8: Difference between revisions
New page: left|200px<br /><applet load="2pi8" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pi8, resolution 2.250Å" /> '''Crystal structure o... |
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[[Image:2pi8.jpg|left|200px]]<br /><applet load="2pi8" size=" | [[Image:2pi8.jpg|left|200px]]<br /><applet load="2pi8" size="350" color="white" frame="true" align="right" spinBox="true" | ||
caption="2pi8, resolution 2.250Å" /> | caption="2pi8, resolution 2.250Å" /> | ||
'''Crystal structure of E. coli MltA with bound chitohexaose'''<br /> | '''Crystal structure of E. coli MltA with bound chitohexaose'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2PI8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | 2PI8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PI8 OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: double-psi beta-barrel; protein-sugar complex; lytic transglycosylase]] | [[Category: double-psi beta-barrel; protein-sugar complex; lytic transglycosylase]] | ||
[[Category: hydrolase]] | |||
''Page seeded by [http:// | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:57:01 2008'' | ||
Revision as of 12:57, 23 January 2008
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Crystal structure of E. coli MltA with bound chitohexaose
OverviewOverview
Crystal structures of an inactive mutant (D308A) of the lytic, transglycosylase MltA from Escherichia coli have been determined in two, different apo-forms, as well as in complex with the substrate analogue, chitohexaose. The chitohexaose binds with all six saccharide residues in, the active site groove, with an intact glycosidic bond at the bond, cleavage center. Its binding induces a large reorientation of the two, structural domains in MltA, narrowing the active site groove and allowing, tight interactions of the oligosaccharide with residues from both domains., The structures identify residues in MltA with key roles in the binding and, recognition of peptidoglycan and confirm that Asp-308 is the single, catalytic residue, acting as a general acid/base. Moreover, the structures, suggest that catalysis involves a high energy conformation of the scissile, glycosidic linkage and that the putative oxocarbenium ion intermediate is, stabilized by the dipole moment of a nearby alpha-helix.
About this StructureAbout this Structure
2PI8 is a Single protein structure of sequence from Escherichia coli with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structure of Escherichia coli Lytic transglycosylase MltA with bound chitohexaose: implications for peptidoglycan binding and cleavage., van Straaten KE, Barends TR, Dijkstra BW, Thunnissen AM, J Biol Chem. 2007 Jul 20;282(29):21197-205. Epub 2007 May 14. PMID:17502382
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