Isoelectric point: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

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 **An easy way to do this for a protein that has a solved structure: get to the page for the PDB ID at [http://www.pdb.org the RCSB Protein Data Bank]. There in the upper right under 'Dsiplay files', click the 'FASTA Sequence' link and download all chains in FASTA format. Open the text file and block the sequence of the chain of interest (excluding the comment line beginning >) and copy it to the clipboard.
 **Alternatively for a protein with a solved structure, go to the [http://www.ebi.ac.uk/msd-srv/oca/oca-bin/ocamain OCA browser-database] and enter your PDB code in search. or use the link to the OCA on the Proteopedia page of each solved structure. And then at the OCA scroll down to Sequence-derived information (near the bottom), click on the link for the one-letter amino acid sequence for one chain and copy the sequence of the chain to the clipboard.
+**For going from a nucleotide sequence, a number of tools are available for 'virtual' translation:
+***At the [http://expasy.org/tools/dna.html ExPASy - Translate tool] choose 'Compact' for the output format below the box where you paste in your sequence.
+***[http://molbiol-tools.ca/Translation.htm Another online site] lists several tools that may be useful depending on the state of the nucleotide sequence you have available.
 *Second, go to the [http://www.embl-heidelberg.de/cgi/pi-wrapper.pl EMBL WWW Gateway to Isoelectric Point Service], paste your sequence in the box, and press the button.