1scj: Difference between revisions
New page: left|200px<br /> <applet load="1scj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1scj, resolution 2.00Å" /> '''CRYSTAL STRUCTURE O... |
No edit summary |
||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
1SCJ is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SCJ OCA]]. | 1SCJ is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Subtilisin Subtilisin]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62]]. Structure known Active Site: AVE. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SCJ OCA]]. | ||
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Subtilisin]] | |||
[[Category: Berman, H.M.]] | [[Category: Berman, H.M.]] | ||
[[Category: Jain, S.C.]] | [[Category: Jain, S.C.]] | ||
| Line 21: | Line 22: | ||
[[Category: subtilisin e - propeptide]] | [[Category: subtilisin e - propeptide]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:05:52 2007'' | ||
Revision as of 12:01, 30 October 2007
|
CRYSTAL STRUCTURE OF SUBTILISIN-PROPEPTIDE COMPLEX
OverviewOverview
We report here the crystallographic structure determination of an, autoprocessed (Ser221Cys)-subtilisin E-propeptide complex at 2.0 A, resolution. The subtilisin domain sequence has a single substitution, (Ser221Cys) which has been shown to block the maturation process prior to, degradation of the propeptide domain (77 residues) that acts as an, intramolecular chaperon. This mutation, however, did not prevent the, enzyme from cleaving its propeptide domain with a 60-80% efficiency. The, current determination is the first example of a subtilisin E-propeptide, complex which has been autoprocessed. A previous structure determination, of a BPN'-prosegment complex has been reported in which the subtilisin, domain was extensively mutated and a calcium binding loop was deleted., Further, in this ... [(full description)]
About this StructureAbout this Structure
1SCJ is a [Protein complex] structure of sequences from [Bacillus subtilis] with CA as [ligand]. Active as [Subtilisin], with EC number [3.4.21.62]. Structure known Active Site: AVE. Full crystallographic information is available from [OCA].
ReferenceReference
The crystal structure of an autoprocessed Ser221Cys-subtilisin E-propeptide complex at 2.0 A resolution., Jain SC, Shinde U, Li Y, Inouye M, Berman HM, J Mol Biol. 1998 Nov 20;284(1):137-44. PMID:9811547
Page seeded by OCA on Tue Oct 30 11:05:52 2007