1tls: Difference between revisions
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==About this Structure== | ==About this Structure== | ||
1TLS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with UFP and C2F as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TLS OCA]]. | 1TLS is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with UFP and C2F as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45]]. Structure known Active Sites: S1 and S2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TLS OCA]]. | ||
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thymidylate synthase]] | |||
[[Category: Hyatt, D.C.]] | [[Category: Hyatt, D.C.]] | ||
[[Category: Maley, F.]] | [[Category: Maley, F.]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
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Revision as of 11:59, 30 October 2007
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THYMIDYLATE SYNTHASE TERNARY COMPLEX WITH FDUMP AND METHYLENETETRAHYDROFOLATE
OverviewOverview
Two crystal structures for E. coli thymidylate synthase (TS) bound to the, mechanism-based inhibitor 5-fluoro-dUMP (FdUMP) and, methylenetetrahydrofolate (CH2THF) have been determined to 2.6 and 2.2 A, nominal resolutions, with crystallographic R factors of 0.180 and 0.178, respectively. The inhibitor and cofactor are well ordered in both, structures and display covalent links to each other and to Cys 146 in the, TS active site. The structures are in general agreement with a previous, report for this complex (D. A. Matthews et al. (1990) J. Mol. Biol. 214, 937-948), but differ in two key respects: (i) the methylene bridge linking, FdUMP and CH2THF is rotated about 60 degrees to a different position and, (ii) the electron density for C6 of FdUMP, which is covalently linked to, Cys 146, is ... [(full description)]
About this StructureAbout this Structure
1TLS is a [Single protein] structure of sequence from [Escherichia coli] with UFP and C2F as [ligands]. Active as [Thymidylate synthase], with EC number [2.1.1.45]. Structure known Active Sites: S1 and S2. Full crystallographic information is available from [OCA].
ReferenceReference
Use of strain in a stereospecific catalytic mechanism: crystal structures of Escherichia coli thymidylate synthase bound to FdUMP and methylenetetrahydrofolate., Hyatt DC, Maley F, Montfort WR, Biochemistry. 1997 Apr 15;36(15):4585-94. PMID:9109668
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