Sandbox 250: Difference between revisions
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Two of the 14 aromatic residues lining the active site gorge that are considered significant for holding ACh in an optimal position for hydrolysis are trp84 and phe330. These residues form the <scene name='Sandbox/Ache_ach/16'>Catalytic Anionic Site</scene>. | Two of the 14 aromatic residues lining the active site gorge that are considered significant for holding ACh in an optimal position for hydrolysis are trp84 and phe330. These residues form the <scene name='Sandbox/Ache_ach/16'>Catalytic Anionic Site</scene>. | ||
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The residues <scene name='Sandbox_250/Ache_fas2/3'>Thr8, Met33, Arg27 and Val34</scene> are present on two loops of the toxin FAS-II. | The residues <scene name='Sandbox_250/Ache_fas2/3'>Thr8, Met33, Arg27 and Val34</scene> are present on two loops of the toxin FAS-II. | ||
When FAS-II <scene name=' | When FAS-II <scene name='Sandbox_250/Ache_fas2/5'>binds</scene> to AChE, Arg27 and Met33 interact with trp279 of the Peripheral Anionic Site, while Val34 and Thr8 interact with tyr70 of the Peripheral Anionic Site. Once this interaction occurs, the entrance of the gorge is <scene name='Sandbox_250/Ache_fas2/1'>covered</scene> such that acetylcholine may not enter. | ||