2uvu: Difference between revisions
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[[Image:2uvu. | [[Image:2uvu.jpg|left|200px]]<br /><applet load="2uvu" size="450" color="white" frame="true" align="right" spinBox="true" | ||
<applet load="2uvu" size="450" color="white" frame="true" align="right" spinBox="true" | |||
caption="2uvu, resolution 2.70Å" /> | caption="2uvu, resolution 2.70Å" /> | ||
'''CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS'''<br /> | '''CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
2UVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with CA and DGT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | 2UVU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with CA and DGT as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Known structural/functional Site: <scene name='pdbsite=AC1:Ca Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2UVU OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: translesion dna polymerase]] | [[Category: translesion dna polymerase]] | ||
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Revision as of 21:07, 18 December 2007
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CRYSTAL STRUCTURES OF MUTANT DPO4 DNA POLYMERASES WITH 8-OXOG CONTAINING DNA TEMPLATE-PRIMER CONSTRUCTS
OverviewOverview
Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4) has been shown to, catalyze bypass of 7,8-dihydro-8-oxodeoxyguanosine (8-oxoG) in a highly, efficient and relatively accurate manner. Crystal structures have revealed, a potential role for Arg(332) in stabilizing the anti conformation of the, 8-oxoG template base by means of a hydrogen bond or ion-dipole pair, which, results in an increased enzymatic efficiency for dCTP insertion and makes, formation of a Hoogsteen pair between 8-oxoG and dATP less favorable., Site-directed mutagenesis was used to replace Arg(332) with Ala, Glu, Leu, or His in order to probe the importance of Arg(332) in accurate and, efficient bypass of 8-oxoG. The double mutant Ala(331)Ala(332) was also, prepared to address the contribution of Arg(331). Transientstate kinetic, results suggest that Glu(332) retains fidelity against bypass of 8-oxoG, that is similar to wild type Dpo4, a result that was confirmed by tandem, mass spectrometric analysis of full-length extension products. A crystal, structure of the Dpo4 Glu(332) mutant and 8-oxoG:C pair revealed, water-mediated hydrogen bonds between Glu(332) and the O-8 atom of 8-oxoG., The space normally occupied by Arg(332) side chain is empty in the crystal, structures of the Ala(332) mutant. Two other crystal structures show that, a Hoogsteen base pair is formed between 8-oxoG and A in the active site of, both Glu(332) and Ala(332) mutants. These results support the view that a, bond between Arg(332) and 8-oxoG plays a role in determining the fidelity, and efficiency of Dpo4-catalyzed bypass of the lesion.
About this StructureAbout this Structure
2UVU is a Single protein structure of sequence from Sulfolobus solfataricus with CA and DGT as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Hydrogen bonding of 7,8-dihydro-8-oxodeoxyguanosine with a charged residue in the little finger domain determines miscoding events in Sulfolobus solfataricus DNA polymerase Dpo4., Eoff RL, Irimia A, Angel KC, Egli M, Guengerich FP, J Biol Chem. 2007 Jul 6;282(27):19831-43. Epub 2007 Apr 27. PMID:17468100
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- DNA-directed DNA polymerase
- Single protein
- Sulfolobus solfataricus
- Egli, M.
- Irimia, A.
- CA
- DGT
- 7
- 8-dihydro-8-oxodeoxyguanosine
- Dna damage
- Dna repair
- Dna replication
- Dna- binding
- Dna-binding
- Dna-directed dna polymerase
- Magnesium
- Metal- binding
- Metal-binding
- Mutator protein
- Nucleotidyltransferase
- P2 dna polymerase iv
- Transferase
- Translesion dna polymerase